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Volume 63, Issue Pt_6

sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH)-contaminated dumpsite Free

Abstract

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped and light-yellow-pigmented bacterium, designated DS15, was isolated from a soil sample collected from a hexachlorocyclohexane dumpsite in Lucknow, Uttar Pradesh, India. Strain DS15 showed highest 16S rRNA gene sequence similarity to DCY34 (98.7 %) and BL03 (98.0 %). The 16S rRNA gene sequence similarity between strain DS15 and species of genus with validly published names ranged from 92.5 % to 98.7 %. The DNA G+C content of strain DS15 was 67.5 mol%. The chemotaxonomic markers in strain DS15 were consistent with its classification in the genus , i.e. Q-10 as the major ubiquinone and summed feature 8 (Cω7/Cω9), Cω6, summed feature 3 (Cω7/Cω6), C 2-OH, C2-OH, C and Cω8 as the predominant fatty acids. The major polar lipids of strain DS15 were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidylglycerol (PG) and sphingoglycolipids (SGL) and spermidine was detected as the major polyamine. Phylogenetic analysis, DNA–DNA hybridization, and chemotaxonomic and phenotypic analysis support the conclusion that strain DS15 represents a novel species within the genus , for which the name is proposed. The type strain is DS15 ( = MTCC 9455 = CCM 7542 = MCC 2023).

  • Published Online:
Funding
This study was supported by the:
  • University of Delhi – Department of Science and Technology (DU/DST)
  • Department of Biotechnology (DBT)
  • Government of India
  • National Bureau of Agriculturally Important Micro-organisms (NBAIM)
  • DBT
  • Council of Scientific and Industrial Research (CSIR), Government of India
© 2013 IUMS
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2013-06-01
2024-04-25
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Sphingopyxis indica sp. nov., isolated from a high dose point hexachlorocyclohexane (HCH)-contaminated dumpsite
Int J Syst Evol Microbiol 63, 2186 (2013); https://doi.org/10.1099/ijs.0.040840-0
/content/journal/ijsem/10.1099/ijs.0.040840-0
/content/journal/ijsem/10.1099/ijs.0.040840-0
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