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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strain, BB-Mw22, was isolated from a tidal flat sediment of the South Sea in South Korea. It grew optimally at 30–37 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-Mw22 belonged to the genus and the cluster comprising species and strain BB-Mw22 was clearly separated from other taxa. Strain BB-Mw22 exhibited 95.3–98.7 % 16S rRNA gene sequence similarity to the type strains of recognized species. Strain BB-Mw22 contained Q-8 as the predominant ubiquionone and iso-C and iso-C 3-OH as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The DNA G+C content of strain BB-Mw22 was 48.9 mol%, and its mean DNA–DNA hybridization values with YCS-5, JCM 16211 and JCM 17727 were 14–28 %. Phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain BB-Mw22 is distinguishable from all recognized species. On the basis of the data presented, strain BB-Mw22 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BB-Mw22 ( = KCTC 23892  = CCUG 62217).

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award 11-2008-00-002-00)
  • Ministry of Education, Science and Technology
  • MEST
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2013-03-01
2024-12-08
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References

  1. Ahn J., Park J.-W., McConnell J. A., Ahn Y.-B., Häggblom M. M. ( 2011 ). Kangiella spongicola sp. nov., a halophilic marine bacterium isolated from the sponge Chondrilla nucula . . Int J Syst Evol Microbiol 61, 961964. [View Article] [PubMed]
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  5. Jean W. D., Huang S.-P., Chen J.-S., Shieh W. Y. ( 2012 ). Kangiella taiwanensis sp. nov. and Kangiella marina sp. nov., marine bacteria isolated from shallow coastal water. . Int J Syst Evol Microbiol 62, 22292234. [View Article] [PubMed]
    [Google Scholar]
  6. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  7. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  8. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  9. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  10. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  11. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. ( 2010 ). Kangiella japonica sp. nov., isolated from a marine environment. . Int J Syst Evol Microbiol 60, 25832586. [View Article] [PubMed]
    [Google Scholar]
  12. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  13. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  14. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  15. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  18. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 5357. [View Article] [PubMed]
    [Google Scholar]
  19. Yoon J.-H., Oh T.-K., Park Y.-H. ( 2004 ). Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 54, 18291835. [View Article] [PubMed]
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Lee S.-Y., Lee J.-S., Oh T.-K. ( 2012 ). Kangiella geojedonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 62, 511514. [View Article] [PubMed]
    [Google Scholar]
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