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Abstract

A Gram-staining-negative, dark orange, strictly aerobic bacterium, designated strain HP12, was isolated from a tidal flat at Hampyeong in South Korea. Cells were moderately halotolerant, catalase- and oxidase-positive, non-motile rods. Growth was observed at 5–35 °C (optimum, 25 °C), at pH 6.0–8.5 (optimum, pH 7.0–7.5), and in the presence of 1–6 % (w/v) NaCl (optimum, 1–2 %). The major cellular fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), iso-C 3-OH, C, iso-C G and iso-C. The polar lipid pattern indicated the presence of phosphatidylethanolamine and two unidentified lipids. The G+C content of the genomic DNA was 37.1 mol% and the predominant respiratory quinone was menaquinone-6. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain formed a tight phyletic lineage with members of the genus and was most closely related to KMM 3961, KMM 3674 and KMM 6032, with 16S rRNA gene sequence similarities of 98.1 %, 98.0 % and 97.8 %, respectively. However, the DNA–DNA relatedness values between strain HP12 and JCM 13509, KCTC 12362 and KCTC 22013 were only 20.2±0.3 %, 22.6±0.6 % and 9.1±2.6 %, respectively. On the basis of phenotypic and molecular features, strain HP12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HP12 ( = KACC 16193 = JCM 17788).

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2013-02-01
2019-10-23
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