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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_3

gen. nov., sp. nov., isolated from the southern North Sea Free

Abstract

A heterotrophic, Gram-stain-negative, aerobic bacterium, designated strain SH4-1, was obtained from a seawater sample collected from the southern North Sea during a phytoplankton bloom. The 16S rRNA gene sequence comparison revealed affiliation to the clade (class ) with SW-265 as the most closely related characterized strain, showing 97.2 % 16S rRNA gene sequence similarity. Calculation of phylogenetic trees based on 16S rRNA gene sequences indicated, however, that members of the genus , Och 114 and Och 149 (95 % and 96 % sequence similarity, respectively) fall between strain SH4-1 and the cluster including HEL-45 (≥95.4 % sequence similarity). Cells of strain SH4-1 are irregular rods with at least one flagellum. Optimal growth occurred between 28 and 32 °C and at a pH between 7.0 and 8.5. Cells require the vitamin nicotinic acid amide as well as sodium ions for growth. The DNA G+C content was 55.1 mol%. The fatty acids (>1 %) comprised C 3-OH, C, C 3-OH, C, C C, Cω7 and 11-methyl Cω7. The polar lipid pattern indicated the presence of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid, one unidentified phospholipid and one other unidentified lipid. On the basis of phenotypic, chemotaxonomic and phylogenetic differences, strain SH4-1 represents a novel species in a new genus within the family , for which we propose the name gen. nov., sp. nov. The type strain of the type species is SH4-1 ( = DSM 23678 = LMG 26343 = CIP 110297).

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Funding
This study was supported by the:
  • Lower Saxony Volkswagen Foundation (VW-Vorab)
© 2013 IUMS
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2013-03-01
2025-11-08

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Pelagimonas varians gen. nov., sp. nov., isolated from the southern North Sea
Int J Syst Evol Microbiol 63, 835 (2013); https://doi.org/10.1099/ijs.0.040675-0
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