1887

Abstract

Three Gram-negative, stalked, motile bacteria, designated 0C-2-2, 0C-17 and LNM-3, were isolated from the red alga . 16S rRNA gene sequence analysis revealed that the three novel strains belonged to the family , and were closely related to G5 (96.5 % 16S rRNA gene sequence similarity) and 26III/A02/215 (94.3 %). The DNA G+C contents of the novel isolates (58.5–60.2 mol%) were clearly distinguished from those of G5 (47.1 mol%) and DSM 19091 (47.9 mol%). The G+C content of G5 obtained in this study was quite different from a previous report (63.6 mol%). DNA–DNA hybridization experiments showed that the novel strains constituted a single species. Eleven phenotypic features of the three isolates differed from those of both related genera. The predominant respiratory quinone was ubiquinone-10 and the major fatty acid was Cω7. On the basis of this polyphasic taxonomic analysis, the novel strains represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is 0C-2-2 ( = LMG 26424  = NBRC 108216).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.040485-0
2013-01-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/1/314.html?itemId=/content/journal/ijsem/10.1099/ijs.0.040485-0&mimeType=html&fmt=ahah

References

  1. Akagawa-Matsushita M. , Itoh T. , Katayama Y. , Kuraishi H. , Yamasato K. . ( 1992; ). Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species. . J Gen Microbiol 138:, 2275–2281. [CrossRef]
    [Google Scholar]
  2. Alain K. , Tindall B. J. , Intertaglia L. , Catala P. , Lebaron P. . ( 2008; ). Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. . Int J Syst Evol Microbiol 58:, 2511–2519. [CrossRef] [PubMed]
    [Google Scholar]
  3. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  4. Baumann L. , Baumann P. , Mandel M. , Allen R. D. . ( 1972; ). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110:, 402–429.[PubMed]
    [Google Scholar]
  5. Biebl H. , Allgaier M. , Tindall B. J. , Koblizek M. , Lünsdorf H. , Pukall R. , Wagner-Döbler I. . ( 2005; ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. . Int J Syst Evol Microbiol 55:, 1089–1096. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. . ( 2009; ). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Fukui Y. , Abe M. , Kobayashi M. , Ishihara K. , Oikawa H. , Yano Y. , Satomi M. . ( 2012; ). Maritalea porphyrae sp. nov., isolated from a red alga (Porphyra yezoensis), and transfer of Zhangella mobilis to Maritalea mobilis comb. nov.. Int J Syst Evol Microbiol 62:, 43–48. [CrossRef] [PubMed]
    [Google Scholar]
  9. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2005; ). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, p. 161. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  10. Ikemoto S. , Katoh K. , Komagata K. . ( 1978; ). Cellular fatty acid composition in methanol-utilizing bacteria. . J Gen Appl Microbiol 24:, 41–49. [CrossRef]
    [Google Scholar]
  11. Johnson J. L. . ( 1981; ). Genetic characterization. . In Manual of Methods for General Bacteriology, pp. 450–472. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. B. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  12. Jung J. Y. , Kim J. M. , Jin H. M. , Kim S. Y. , Park W. , Jeon C. O. . ( 2011; ). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61:, 1534–1538. [CrossRef] [PubMed]
    [Google Scholar]
  13. Justé A. , Van Trappen S. , Verreth C. , Cleenwerck I. , De Vos P. , Lievens B. , Willems K. A. . ( 2012; ). Characterization of Tetragenococcus strains from sugar thick juice reveals a novel species, Tetragenococcus osmophilus sp. nov., and divides Tetragenococcus halophilus into two subspecies, T. halophilus subsp. halophilus subsp. nov. and T. halophilus subsp. flandriensis subsp. nov.. Int J Syst Evol Microbiol 62:, 129–137. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  15. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  16. Lee K.-B. , Liu C.-T. , Anzai Y. , Kim H. , Aono T. , Oyaizu H. . ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55:, 1907–1919. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lee K. , Lee H. K. , Choi T.-H. , Cho J.-C. . ( 2007; ). Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. . Int J Syst Evol Microbiol 57:, 2595–2599. [CrossRef] [PubMed]
    [Google Scholar]
  18. Luthria D. L. , Sprecher H. . ( 1993; ). 2-Alkenyl-4,4-dimethyloxazolines as derivatives for the structural elucidation of isomeric unsaturated fatty acids. . Lipids 28:, 561–564. [CrossRef] [PubMed]
    [Google Scholar]
  19. Martens T. , Heidorn T. , Pukall R. , Simon M. , Tindall B. J. , Brinkhoff T. . ( 2006; ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef] [PubMed]
    [Google Scholar]
  20. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  21. Ogata E. . ( 1970; ). On a new algal culture medium SWM-III. . Bull Jpn Soc Phycol 18:, 171–173 (in Japanese).
    [Google Scholar]
  22. Satomi M. , Kimura B. , Mizoi M. , Sato T. , Fujii T. . ( 1997; ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. . Int J Syst Bacteriol 47:, 832–836. [CrossRef] [PubMed]
    [Google Scholar]
  23. Sawabe T. , Oda Y. , Shiomi Y. , Ezura Y. . ( 1995; ). Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. . Microb Ecol 30:, 193–202. [CrossRef]
    [Google Scholar]
  24. Schlesner H. , Bartels C. , Sittig M. , Dorsch M. , Stackebrandt E. . ( 1990; ). Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov.. Int J Syst Bacteriol 40:, 443–451. [CrossRef] [PubMed]
    [Google Scholar]
  25. Stackebrandt E. , Liesack W. . ( 1993; ). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 151–194. Edited by Goodfellow M. , O’Donnell A. G. . . London:: Academic Press;.
    [Google Scholar]
  26. Strömpl C. , Hold G. L. , Lünsdorf H. , Graham J. , Gallacher S. , Abraham W.-R. , Moore E. R. B. , Timmis K. N. . ( 2003; ). Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. . Int J Syst Evol Microbiol 53:, 1901–1906. [CrossRef] [PubMed]
    [Google Scholar]
  27. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  28. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  30. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  31. Weiner R. M. , Devine R. A. , Powell D. M. , Dagasan L. , Moore R. L. . ( 1985; ). Hyphomonas oceanitis sp. nov., Hyphomonas hirschiana sp. nov., and Hyphomonas jannaschiana sp. nov.. Int J Syst Bacteriol 35:, 237–243. [CrossRef]
    [Google Scholar]
  32. Weiner R. M. , Melick M. , O’Neill K. , Quintero E. . ( 2000; ). Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. . Int J Syst Evol Microbiol 50:, 459–469. [CrossRef] [PubMed]
    [Google Scholar]
  33. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.040485-0
Loading
/content/journal/ijsem/10.1099/ijs.0.040485-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error