1887

Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, D1-y4, was isolated from a tidal flat sediment of the South Sea in South Korea and subjected to a polyphasic study. Strain D1-y4 grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-y4 belonged to the genus , joining the type strain of , with which it exhibited 97.8 % similarity. Sequence similarities to SMK-12 and KYW382 were 96.5 and 96.2 %, respectively. Strain D1-y4 contained MK-6 as the predominant menaquionone and iso-C, iso-C G and anteiso-C as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain D1-y4 was 34.6 mol% and its mean DNA–DNA relatedness value with . KCTC 23046 was 7 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain D1-y4 is distinguishable from the three recognized species. On the basis of the data presented here, strain D1-y4 is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is D1-y4 ( = KCTC 23716  = CCUG 61504).

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award 11-2008-00-002-00)
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • Ministry of Education, Science and Technology
  • MEST
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2013-03-01
2024-10-13
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