A Gram-negative, orange-pigmented, rod-shaped, motile and aerobic bacterial strain designated DM9T was isolated from hexachlorocyclohexane (HCH)-contaminated soil (Lucknow, India) and its taxonomic position was determined using a polyphasic approach. 16S rRNA gene sequence analysis showed that the isolate belonged to the phylum Bacteroidetes and confirmed its placement in the genus Pontibacter, with sequence similarity ranging from 93.92 to 96.21 % with other members of the genus Pontibacter. The major cellular fatty acids of the novel strain were iso-C17 : 0 3-OH (6.00 %), iso-C15 : 0 (21.54 %) and summed feature 4 (comprising C17 : 1 iso I/anteiso B; 32.3 %). The polar lipid profile of strain DM9T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, two unknown aminolipids and four unknown polar lipids. Strain DM9T contained MK-7 as the predominant menaquinone and its DNA G+C content was 49.2 mol%. sym-Homospermidine was the major polyamine observed in the cell. The results obtained on the basis of phenotypic characteristics, phylogenetic analysis, biochemical and physiological tests clearly distinguished DM9T from closely related members of the genus Pontibacter. It is proposed that DM9T represents a novel species, Pontibacter lucknowensis sp. nov.; the type strain is DM9T ( = CCM 7955T = MTCC 11079T).
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Funding
This study was supported by the:
Department of Biotechnology, National Bureau of Agriculturally Important Micro-organisms, Indian Council of Agricultural Research
Arden-JonesM. P.,
McCarthyA. J.,
CrossT.(1979). Taxonomic and serologic studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula
. . J Gen Microbiol115, 343–354. [View Article][PubMed]
BowmanJ. P.,
NicholsC. M.,
GibsonJ. A. E.(2003).Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. . Int J Syst Evol Microbiol53, 1343–1355. [View Article][PubMed]
ChristensenW. B.(1946). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. . J Bacteriol52, 461–466.
ChunJ.,
LeeJ. H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol57, 2259–2261. [View Article][PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100, 221–230. [View Article][PubMed]
GonzalezJ. M.,
Saiz-JimenezC.(2002). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol4, 770–773. [View Article][PubMed]
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24, 54–63. . [View Article]
JoungY.,
KimH.,
AhnT. S.,
JonK.(2011).Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea. . J Microbiol49, 290–293. [View Article][PubMed]
JukesT.,
CantorC. R.(1969).Evolution of Protein Molecules in Mammalian Protein Metabolism, pp. 21–132. Edited by
MunroH. N.
. New York:: Academic Press;. [CrossRef]
KaurJ.,
VermaM.,
LalR.(2011).Rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of Blastobacter aggregatus Hirsch and Muller 1986 as Rhizobium aggregatum comb. nov.. Int J Syst Evol Microbiol61, 1218–1225. [View Article][PubMed]
KumariR.,
SubudhiS.,
SuarM.,
DhingraG.,
RainaV.,
DograC.,
LalS.,
van der MeerJ. R.,
HolligerC.,
LalR.(2002). Cloning and characterization of lin genes responsible for the degradation of hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90. . Appl Environ Microbiol68, 6021–6028. [View Article][PubMed]
KuykendallL. D.,
RoyM. A.,
O’NeillJ. J.,
DevineT. E.(1988). Fatty acids, antibiotics resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum
. . Int J Syst Bacteriol38, 358–361. [View Article]
NedashkovskayaO. I.,
KimS. B.,
SuzukiM.,
ShevchenkoL. S.,
LeeM. S.,
LeeK. H.,
ParkM. S.,
FrolovaG. M.,
OhH. W.& other authors (2005).Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol55, 2583–2588. [View Article][PubMed]
PrakashO.,
KumariK.,
LalR.(2007).Pseudomonas delhiensis sp. nov., isolated from fly ash dumping site of thermal power plant, Delhi, India. . Int J Syst Evol Microbiol57, 527–531. [View Article][PubMed]
SmibertR. M.,
KriegN. R.(1994). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
SureshK.,
MayilrajS.,
ChakrabartiT.(2006).Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’. . Int J Syst Evol Microbiol56, 1703–1707. [View Article][PubMed]
Van de PeerY.,
De WachterR.(1994). TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. . Comput Appl Biosci10, 569–570.[PubMed]