1887

Abstract

Two novel chemo-organoheterotrophic members of the were isolated from alpine and pre-alpine lakes. Cells stained Gram-negative, were motile and rod-shaped, and formed yellow, circular, convex colonies on different agar media. Strains 301 and 469 were strictly aerobic, catalase- and oxidase-positive, and grew at temperatures between 10 and 40 °C (optimum, 28 °C), and at pH values between 5 and 10 (optimum, pH 7). Both strains contained Q-10 as the dominant quinone, sphingoglycolipids and 2-hydroxymyristic acid, whereas 3-hydroxy fatty acids were absent. Major fatty acids of strain 301 were Cω7 (53.3 %) and Cω7 (22.9 %), with C 2-OH (10.8 %) as the major 2-hydroxy fatty acid. Fatty acids of strain 469 were dominated by Cω7 (34.4 %), Cω7c (32.0 %) and C 2-OH (15.2 %) as the major 2-hydroxy fatty acid. The genomic DNA G+C contents of strains 301 and 469 were 63.4 and 64.6 mol%, respectively. 16S rRNA gene sequence comparison indicated that both strains belonged to the genus . This classification was supported by the presence of spermidine as the major polyamine. The phylogenetically closest relatives of strain 301 were DSM 16289, DSM 21299, DSM 7462 and DSM 19371 (98.8, 98.0, 97.9 and 97.4 % sequence similarity, respectively). DNA–DNA hybridization of genomic DNA yielded similarities in the range 43.2–12.1 % between strain 301 and the type strains of these four species. Closest relatives of strain 469 were DSM 7465 and DSM 19371 (97.1 and 96.5 % 16S rRNA gene sequence similarity, respectively). The degree of DNA–DNA hybridization between strain 469 and DSM 7465 was 17.9 %. Based on the results of the molecular analyses and their phenotypic characteristics, strains 301 and 469 represent two novel species of the genus . The name sp. nov. is proposed for strain 301( = DSM25076 = LMG 26659). The name sp. nov. is proposed for strain 469 ( = DSM 25079 = LMG 26901). The polyphasic analysis also suggests that should be reclassified as comb. nov. with Ca1 ( = EY 2404 = ATCC 49355 = CIP 105429 = DSM 7465 = ICMP 12535 = NBRC 15211 = JCM 8521 = LMG 17323 = NCPPB 3629) as the type strain.

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2013-02-01
2020-01-20
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