1887

Abstract

Five nocardioform isolates from human clinical sources were evaluated. Analysis of the nearly full-length 16S rRNA gene showed 99.9–100 % similarity among the strains. The results of a comparative phylogenetic analysis of the 16S rRNA gene sequences indicated that the isolates belonged to the genus . Phenotypic and molecular analyses were performed on the clinical isolates. Traditional phenotypic analyses included morphological, biochemical/physiological, chemotaxonomic and antimicrobial susceptibility profiling. Molecular studies included 1441-bp 16S rRNA and 1246-bp gene sequence analyses, as well as DNA–DNA hybridizations. Biochemical analysis failed to differentiate the putative novel species from its phylogenetic neighbours; however, molecular studies were able to distinguish the patient strains and confirm them as members of a single species. Based on 16S rRNA gene sequence analysis, similarity between the isolates and their closest relatives (type strains of , , and ) was ≤99.3 %. Analysis of partial gene sequences showed 98–99.7 % relatedness among the isolates. and were the closest related species to the isolates based on gene sequence analysis, and their type strains showed 95.7 and 95.3 % similarity, respectively, to strain W9988. Resistance to amikacin and molecular analyses, including DNA–DNA hybridization, distinguished the five patient strains from their phylogenetic neighbours, and the results of this polyphasic study indicated the existence of a novel species of , for which we propose the name sp. nov., with strain W9988 ( = DSM 45539  = CCUG 59655) as the type strain.

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2013-03-01
2019-10-15
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References

  1. Baba T., Nishiuchi Y., Yano I.. ( 1997;). Composition of mycolic acid species as a criterion in nocardial classification. . Int J Syst Bacteriol 47:, 795–801. [CrossRef]
    [Google Scholar]
  2. Berd D.. ( 1973;). Laboratory identification of clinically important aerobic actinomycetes. . Appl Microbiol 25:, 665–681.[PubMed]
    [Google Scholar]
  3. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G.. ( 1982;). Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. . J Clin Microbiol 15:, 1133–1140.[PubMed]
    [Google Scholar]
  4. Brown-Elliott B. A., Brown J. M., Conville P. S., Wallace R. J. Jr. ( 2006;). Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. . Clin Microbiol Rev 19:, 259–282. [CrossRef][PubMed]
    [Google Scholar]
  5. CLSI ( 2003;). Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes. Approved Standard M24-A. Wayne, PA: Clinical and Laboratory Standards Institute.
  6. Conville P. S., Witebsky F. G.. ( 2007;). Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and other aerobic actinomycetes. . In Manual of Clinical Microbiology, , 9th edn., pp. 515–542. Edited by Murray P. R., Baron E. J., Jorgensen J. H., Landry M. L., Pfaller M. A... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Conville P. S., Brown J. M., Steigerwalt A. G., Brown-Elliott B. A., Witebsky F. G.. ( 2008;). Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the “Nocardia transvalensis complex”. . J Clin Microbiol 46:, 1178–1184. [CrossRef][PubMed]
    [Google Scholar]
  8. DeCroos F. C., Garg P., Reddy A. K., Sharma A., Krishnaiah S., Mungale M., Mruthyunjaya P..Hyderabad Endophthalmitis Research Group ( 2011;). Optimizing diagnosis and management of nocardia keratitis, scleritis, and endophthalmitis: 11-year microbial and clinical overview. . Ophthalmology 118:, 1193–1200. [CrossRef][PubMed]
    [Google Scholar]
  9. Goodfellow M.. ( 1998;). Nocardia and related genera. . In Topley and Wilson’s Microbiology and Microbial Infections, , 9th edn., vol. 2, pp. 463–489. Edited by Balows A., Duerden B. I... London:: Arnold;.
    [Google Scholar]
  10. Goodfellow M., Maldonado L. A.. ( 2006;). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. . In The Prokaryotes, , 3rd edn., vol. 3, pp. 843–888. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Buczolits S., Jäckel U., Grün-Wollny I., Busse H.-J.. ( 2004;). Nocardia tenerifensis sp. nov.. Int J Syst Evol Microbiol 54:, 381–383. [CrossRef][PubMed]
    [Google Scholar]
  12. Klatte S., Kroppenstedt R. M., Rainey F. A.. ( 1994;). Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus-species. . Syst Appl Microbiol 17:, 355–360. [CrossRef]
    [Google Scholar]
  13. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver loaded ion exchanger. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  14. Kroppenstedt R. M.. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series no. 20), pp. 173–199. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  15. Lalitha P., Tiwari M., Prajna N. V., Gilpin C., Prakash K., Srinivasan M.. ( 2007;). Nocardia keratitis: species, drug sensitivities, and clinical correlation. . Cornea 26:, 255–259. [CrossRef][PubMed]
    [Google Scholar]
  16. Lasker B. A., Moser B. D., Brown J. M.. ( 2011;). Gordonia. . In Molecular Detection of Human Bacterial Pathogens, pp. 95–110. Edited by Liu D... Boca Raton, FL:: CRC Taylor & Francis Group;. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  18. Loeffelholz M. J., Scholl D. R.. ( 1989;). Method for improved extraction of DNA from Nocardia asteroides. . J Clin Microbiol 27:, 1880–1881.[PubMed]
    [Google Scholar]
  19. Manikandan P., Bhaskar M., Revathi R., Anita R., Abarna Lakshmi L. R., Narendran V.. ( 2007;). Isolation and antimicrobial susceptibility pattern of Nocardia among people with culture-proven ocular infections attending a tertiary care eye hospital in Tamilnadu, South India. . Eye (Lond) 21:, 1102–1108. [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., Patel V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson A., Athalye M., Schaal K. P., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  23. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. ( 1955;). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  26. Shen F.-T., Lu H. L., Lin J. L., Huang W. S., Arun A. B., Young C. C.. ( 2006;). Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene. . Res Microbiol 157:, 367–375. [CrossRef][PubMed]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  29. Weyant R. S., Moss C. W., Weaver R. E., Hollis D. G., Jordan J. G., Cook E. C., Daneshvar M. I.. ( 1996;). Identification of Unusual Pathogenic Gram-Negative Aerobic and Facultatively Anaerobic Bacteria, , 2nd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  30. Wilson R. W., Steingrube V. A., Brown B. A., Blacklock Z., Jost K. C. Jr, McNabb A., Colby W. D., Biehle J. R., Gibson J. L., Wallace R. J. Jr. ( 1997;). Recognition of a Nocardia transvalensis complex by resistance to aminoglycosides, including amikacin, and PCR-restriction fragment length polymorphism analysis. . J Clin Microbiol 35:, 2235–2242.[PubMed]
    [Google Scholar]
  31. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. J., Schaal K. P.. ( 1995;). Tsukamurella inchonensis sp. nov.. Int J Syst Bacteriol 45:, 522–527. [CrossRef][PubMed]
    [Google Scholar]
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