sp. nov., isolated from seawater Free

Abstract

A Gram-negative, rod-shaped, non-motile and pink bacterial strain, designated strain GCM0142, was isolated from the confined seawater in the Saemangeum Tide Embankment of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GCM0142 indicated that the isolate belonged to the phylum and exhibited similarity levels of 94.0–96.4 % to the type strains of recognized species. Strain GCM0142 was oxidase- and catalase-positive. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-CI and/or anteiso-CB, 36.8 %), iso-C (22.3 %) and summed feature 3 (comprising Cω7 and/or Cω6, 6.2 %). The DNA G+C content of strain GCM0142 was 48.9 mol% and the major quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids (AL1–2), an unknown aminophospholipid, five unknown lipids (L1–5) and an unknown glycolipid. On the basis of the evidence presented, strain GCM0142 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GCM0142 ( = KACC 16448 = JCM 17926).

Funding
This study was supported by the:
  • Chonbuk National University, Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.039867-0
2013-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/565.html?itemId=/content/journal/ijsem/10.1099/ijs.0.039867-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  2. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  3. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  4. Dastager S. G., Raziuddin Q. S., Deepa C. K., Li W. J., Pandey A. ( 2010 ). Pontibacter niistensis sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60, 28672870. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  8. Joung Y., Kim H., Ahn T.-S., Jon K. ( 2011 ). Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea. . J Microbiol 49, 290293. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  11. Minnikin D., O’Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  12. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. & other authors ( 2005 ). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55, 25832588. [View Article] [PubMed]
    [Google Scholar]
  13. Raichand R., Kaur I., Singh N. K., Mayilraj S. ( 2011 ). Pontibacter rhizosphera sp. nov., isolated from rhizosphere soil of an Indian medicinal plant Nerium indicum . . Antonie van Leeuwenhoek 100, 129135. [View Article] [PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  15. Suresh K., Mayilraj S., Chakrabarti T. ( 2006 ). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’. . Int J Syst Evol Microbiol 56, 17031707. [View Article] [PubMed]
    [Google Scholar]
  16. Tamaoka J., Katayama‐Fujimura Y., Kuraishi H. ( 1983 ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Microbiol 54, 3136. [View Article]
    [Google Scholar]
  17. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [PubMed] [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  19. Tittsler R. P., Sandholzer L. A. ( 1936 ). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31, 575580.[PubMed]
    [Google Scholar]
  20. Wang Y., Zhang K., Cai F., Zhang L., Tang Y., Dai J., Fang C. ( 2010 ). Pontibacter xinjiangensis sp. nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov.. Int J Syst Evol Microbiol 60, 99103. [View Article] [PubMed]
    [Google Scholar]
  21. Xu M., Wang Y., Dai J., Jiang F., Rahman E., Peng F., Fang C. ( 2012 ). Pontibacter populi sp. nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest. . Int J Syst Evol Microbiol 62, 665670. [View Article] [PubMed]
    [Google Scholar]
  22. Zhang L., Zhang Q., Luo X., Tang Y., Dai J., Li Y., Wang Y., Chen G., Fang C. ( 2008 ). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 58, 12101214. [PubMed] [CrossRef]
    [Google Scholar]
  23. Zhou Y., Wang X., Liu H., Zhang K. Y., Zhang Y. Q., Lai R., Li W. J. ( 2007 ). Pontibacter akesuensis sp. nov., isolated from a desert soil in China. . Int J Syst Evol Microbiol 57, 321325. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.039867-0
Loading
/content/journal/ijsem/10.1099/ijs.0.039867-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed