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Volume 63, Issue Pt_2

sp. nov., isolated from root nodules of spp. Free

Abstract

Five strains, JPY461, JPY359, JPY389, DPU-3 and STM4206 were isolated from nitrogen-fixing nodules on the roots of spp. and their taxonomic positions were investigated using a polyphasic approach. All five strains grew at 15–40 °C (optimum, 30–37 °C), at pH 4.0–8.0 (optimum, pH 6.0–7.0) and with 0–1 % (w/v) NaCl [optimum, 0 % (w/v)]. On the basis of 16S rRNA gene sequence analysis, a representative strain (JPY461) showed 97.2 % sequence similarity to the closest related species SA33, a similarity of 97.2 % to KMY02, 97.1 % to STM815 and 97.1 % to LMG 20598. The predominant fatty acids of the five novel strains were summed feature 2 (comprising C iso I and/or C 3-OH), summed feature 3 (comprising C 7 and/or C 6), C , C 3-OH, C cyclo, C 7 and C cyclo 8. The major isoprenoid quinone was Q-8 and the DNA G+C content of the strains was 63.0–65.0 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, an unidentified aminolipid and several unidentified phospholipids. The DNA–DNA relatedness of the novel strain with respect to recognized species of the genus was less than 54 %. On the basis of 16S rRNA and gene sequence similarities, chemotaxonomic and phenotypic data, the five strains represent a novel species in the genus , for which the name sp. nov. is proposed with the type strain, JPY461 ( = LMG 26031 = BCRC 80259 = KCTC 23308).

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Funding
This study was supported by the:
  • National Science Council, Taipei, Taiwan, Republic of China (Award NSC 96-2313-B-022-001-MY3)
© 2013 IUMS
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Burkholderia diazotrophica sp. nov., isolated from root nodules of Mimosa spp.
Int J Syst Evol Microbiol 63, 435 (2013); https://doi.org/10.1099/ijs.0.039859-0
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