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Abstract
A Gram-negative, rod-shaped, motile, non-spore-forming and poly-β-hydroxybutyrate-containing bacterial strain, designated NAFc-7T, was isolated from groundwater in Taiwan and was characterized using a polyphasic taxonomic approach. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 6.0–7.0) and with 0–3 % NaCl (optimum, 0–1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NAFc-7T, together with Pseudorhodoferax soli TBEA3T (98.2 % sequence similarity) and Pseudorhodoferax caeni SB1T (98.0 %), formed a deep line within the family Comamonadaceae . Strain NAFc-7T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C17 : 0 cyclo as the predominant fatty acids. The major 3-hydroxy fatty acid was C10 : 0 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine and an uncharacterized phospholipid. The DNA–DNA relatedness of strain NAFc-7T with respect to recognized species of the genus Pseudorhodoferax was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NAFc-7T represents a novel member of the genus Pseudorhodoferax , for which the name Pseudorhodoferax aquiterrae sp. nov. is proposed. The type strain is NAFc-7T ( = BCRC 80210T = LMG 26333T = KCTC 23314T).
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