1887

Abstract

A Gram-negative, rod-shaped, motile, non-spore-forming and poly-β-hydroxybutyrate-containing bacterial strain, designated NAFc-7, was isolated from groundwater in Taiwan and was characterized using a polyphasic taxonomic approach. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 6.0–7.0) and with 0–3 % NaCl (optimum, 0–1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NAFc-7, together with TBEA3 (98.2 % sequence similarity) and SB1 (98.0 %), formed a deep line within the family . Strain NAFc-7 contained summed feature 3 (comprising Cω7 and/or Cω6), C and C cyclo as the predominant fatty acids. The major 3-hydroxy fatty acid was C 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 67.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylserine and an uncharacterized phospholipid. The DNA–DNA relatedness of strain NAFc-7 with respect to recognized species of the genus was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NAFc-7 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is NAFc-7 ( = BCRC 80210 = LMG 26333 = KCTC 23314).

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2013-01-01
2020-08-12
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