RT Journal Article SR Electronic(1) A1 Niharika, Neha A1 Moskalikova, Hana A1 Kaur, Jasvinder A1 Sedlackova, Miroslava A1 Hampl, Ales A1 Damborsky, Jiri A1 Prokop, Zbynek A1 Lal, RupYR 2013 T1 Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_2 SP 667 OP 672 DO https://doi.org/10.1099/ijs.0.039826-0 PB Microbiology Society, SN 1466-5034, AB A yellow-pigmented bacterial strain, designated LL02T, was isolated from hexachlorocyclohexane-contaminated soil from Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02T occupied a distinct phylogenetic position in the genus Novosphingobium and showed the highest sequence similarity with Novosphingobium resinovorum NCIMB 8767T (98.59 %). DNA–DNA relatedness between strain LL02T and its closest phylogenetic neighbours was <70 %, which indicated that strain LL02T represented a novel species of the genus Novosphingobium . The DNA G+C content of strain LL02T was 67.72±0 mol%. The major respiratory quinone was ubiquinone Q-10. The polar lipid profile of the isolate corresponded to those reported for other members of the genus Novosphingobium (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its classification in the genus. Spermidine was the major polyamine. The major fatty acids were summed feature 3 (consisting of C16 : 1ω7c and/or C16 : 1ω6c; 40.13 %), summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c; 31.09 %) and C14 : 0 2-OH (23.16 %). The results obtained from DNA–DNA hybridization and biochemical and physiological tests clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain LL02T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium barchaimii sp. nov. is proposed. The type strain is LL02T ( = CCM 7980T  = DSM 25411T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.039826-0