Two strains (NT29T and NT31T) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29T was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29T ( = BCC 47634T = NBRC 108868T = CBS 12319T). Strain NT31T represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31T ( = BCC 47635T = NBRC 108869T = CBS 12320T).
Published Online:
Funding
This study was supported by the:
Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
CadeteR. M.,
SantosR. O.,
MeloM. A.,
MouroA.,
GonçalvesD. L.,
StambukB. U.,
GomesF. C. O.,
LachanceM. A.,
RosaC. A.2009; Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 9:1338–1342 [View Article][PubMed]
CadeteR. M.,
MeloM. A.,
LopesM. R.,
PereiraG. M. D.,
ZilliJ. E.,
VitalM. J. S.,
GomesF. C. O.,
LachanceM.-R.,
RosaC. A.2012; Candida amazonensis sp. nov., an ascomycetous yeast isolated from rotting wood in Amazonian Forest, Brazil. Int J Syst Evol Microbiol 62:1438–1440 [View Article][PubMed]
CarvalhoW.,
SilvaS. S.,
VitoloM.,
FelipeM. G. A.,
MancilhaI. M.2002; Improvement in xylitol production from sugarcane bagasse hydrolysate achieved by the use of a repeated-batch immobilized cell system. Z Naturforsch C 57:109–112[PubMed][CrossRef]
de GarcíaV.,
BrizzioS.,
LibkindD.,
RosaC. A.,
van BroockM.2010; Wickerhamomyces patagonicus sp. nov., an ascomycetous yeast species from Patagonia, Argentina. Int J Syst Evol Microbiol 60:1693–1696 [View Article][PubMed]
Doran-PetersonJ.,
CookD. M.,
BrandonS. K.2008; Microbial conversion of sugars from plant biomass to lactic acid or ethanol. Plant J 54:582–592 [View Article][PubMed]
Hahn-HägerdalB.,
GalbeM.,
Gorwa-GrauslundM. F.,
LidénG.,
ZacchiG.2006; Bio-ethanol – the fuel of tomorrow from the residues of today. Trends Biotechnol 24:549–556 [View Article][PubMed]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
KuraishiH.,
Katayama-FujimuraY.,
SugiyamaJ.,
YokoyamaT.1985; Ubiquinone systems in fungi. I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395
KurtzmanC. P.2011a; Pachysolen Boidin & Adzet (1957). The Yeasts, a Taxonomic Study, 5th edn. pp. 673–684 Edited by
KurtzmanC. P.,
FellJ. W.,
BoekhoutT.
Amsterdam: Elsevier;
KurtzmanC. P.2011b; Scheffersomyces Kurtzman & M. Suzuki (2010). The Yeasts, a Taxonomic Study, 5th edn. pp. 773–777 Edited by
KurtzmanC. P.,
FellJ. W.,
BoekhoutT.
Amsterdam: Elsevier;
KurtzmanC. P.2011d; Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus
. Antonie van Leeuwenhoek9913–23 [View Article][PubMed]
KurtzmanC. P.,
SuzukiM.2010; Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces
. Mycoscience 51:2–14 [View Article]
KurtzmanC. P.,
RobnettC. J.,
Basehoar-PowersE.2008; Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov.. FEMS Yeast Res 8:939–954 [View Article][PubMed]
LachanceM. A.,
BowlesJ. M.,
StarmerW. T.,
BarkerJ. S.1999; Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45:172–177[PubMed][CrossRef]
LachanceM. A.,
BoekhoutT.,
ScorzettiG.,
FellJ. W.,
KurtzmanC. P.2011; Candida Berkhout. The Yeasts, a Taxonomic Study, 5th edn. pp. 987–1279 Edited by
KurtzmanC. P.,
FellJ. W.,
BoekhoutT.
Amsterdam: Elsevier;
LimtongS.,
YongmanitchaiW.,
TunM. M.,
KawasakiH.,
SekiT.2007; Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422 [View Article][PubMed]
LimtongS.,
YongmanitchaiW.,
KawasakiH.,
FujiyamaK.2009; Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand. FEMS Yeast Res 9:504–510 [View Article][PubMed]
NakaseT.,
SuzukiM.1986; Bullera megalospora, a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinesis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240 [View Article]
O’DonnellK.1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic and Pleomorphic Speciation in Fungal Systematics pp. 225–233 Edited by
ReynoldsD. R.,
TaylorJ. W.
Wallingford, UK: CAB International;
RosaC. A.,
MoraisP. B.,
LachanceM. A.,
SantosR. O.,
MeloW. G. P.,
VianaR. H. O.,
BragançaM. A.,
PimentaR. S.2009; Wickerhamomyces queroliae sp. nov. and Candida jalapaonensis sp. nov., two yeast species isolated from Cerrado ecosystem in North Brazil. Int J Syst Evol Microbiol 59:1232–1236 [View Article][PubMed]
WhiteT. J.,
BrunsT.,
LeeS.,
TaylorJ. W.1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications pp. 315–322 Edited by
InnisM. A.,
GelfandD. H.,
SninskyJ. J.,
WhiteT. J.
New York: Academic Press;
YamadaY.,
KondoK.1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus, and the yeast-like genera Sporobolomyces and Rhodosporidium
. J Gen Appl Microbiol 19:59–77 [View Article]
YarrowD.1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp. 77–100 Edited by
KurtzmanC. P.,
FellJ. W.
Amsterdam: Elsevier; [View Article]