1887

Abstract

The life cycle, prey range and taxonomic status of a -like organism, strain JSS, were studied. Strain JSS was isolated from sewage in London, Ontario, Canada, in enrichment culture with prey cells. During predation, this strain remained attached to the outside of a stalked cell. No periplasmic growth stage was observed and no bdelloplast was formed. The stalked cells of retained their shape and, after predation, were devoid of cytoplasmic content, as shown by transmission electron microscopy. A periplasmic growth stage has been a definitive character in the description of members of the genera , , and . This is the first description of an epibiotic predator in this group of prokaryotic predators. The G+C content of the genomic DNA of strain JSS was 46.1 mol%. 16S rRNA gene sequence analysis showed that this strain was related to strains HD100, 109J, 114 and 127 (90–93 % similarity). Phylogenetic analysis based on 16S rRNA gene sequences grouped strain JSS with the cluster, but at a distance from other isolates. On the basis of features of the life cycle and phylogenetic data, it was concluded that strain JSS merits classification as the type strain of a novel species, for which the name sp. nov. is proposed (type strain JSS  = ATCC BAA-2330  = DSM 25223).

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2013-01-01
2020-01-27
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