1887

Abstract

A short rod-shaped Gram-stain-positive actinobacterium was isolated as an endophyte from the tissues of cv. KB27 and was investigated by means of a polyphasic taxonomic approach. An analysis of its 16S rRNA gene sequence indicated that strain S9-650 forms an individual line of descent and is related to certain members of the suborder , order (<95 % sequence similarity). Distance-matrix and neighbour-joining analyses set the branching point of the novel isolate between two clades, one being represented by members of the genera (family ) and (family ) and the other by members of the genera , and (family ). The organism had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The acyl type was found to be -glycolylated. The major menaquinone was MK-9(H) and the fatty acid profile was characterized by the predominance of iso-C, Cω9, anteiso-C and Cω8 The polar lipids comprised diphosphatidylglycerol, an unidentified glycolipid, phospholipids and aminolipids. The G+C content of the genomic DNA was 71.2 mol%. The distinct phylogenetic position and the phenotypic markers that clearly separate the novel organism from all other members of the suborder indicate that strain S9-650 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is S9-650 ( = DSM 45627 = KACC 16232).

Funding
This study was supported by the:
  • Temasek Foundation
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2013-04-01
2024-12-07
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