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Volume 62, Issue Pt_12

Reclassification and emended description of as comb. nov., and emendation of the genus Free

Abstract

‘’ DSM 4733 has been shown to be affiliated with the family instead of the , and due to its poor characterization has been omitted from the current edition of and removed to limbo. We isolated a novel sphingoglycolipid-containing dimorphic prosthecate bacterium, designated strain 247, from a pre-alpine freshwater lake. Strain 247 and ‘ DSM 4733 were characterized in detail. The rod-shaped cells were Gram-stain-negative, aerobic, catalase- and oxidase-positive, and formed a stalk or polar flagellum. Both strains grew optimally at 28–30 °C, and pH 6.0–8.0. The major fatty acids were Cω7, C and 11-methyl Cω7. C 2-OH represents the major 2-hydroxy fatty acid. Q-10 was the major respiratory quinone and the major polar lipids were diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, three glycolipids, two phosphoaminolipids and two unidentified sphingoglycolipids. The major polyamine was -homospermidine. The G+C content of genomic DNA of strains 247 and DSM 4733 was 67.6 mol% and 67.0 mol%, respectively. According to 16S rRNA gene sequence analysis and DNA–DNA hybridization, strains DSM 4733 and 247 were phylogenetically closely related (99.6 % 16S rRNA gene sequence similarity, 82.9 % DNA–DNA hybridization value) and affiliated to the genus . The closest recognized species was DSM 15581 (98.1 % sequence similarity). In addition, the presence of cystine arylamidase, absence of β-galactosidase, and the inability to utilize -arabinose, galactose and sucrose distinguished strains DSM 4733 and 247 from most other members of the family . So far, the dimorphic life cycle that involves a prosthecate and a flagellated stage is unique for strains DSM 4733 and 247 among all members of the family . Therefore, is reclassified as , with the type strain DSM 4733 ( = ATCC 15260 = CIP 106443 = VKM B-1368) and strain 247 (DSM 25078 = LMG 26658) as an additional strain of this species.

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Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft (Award OV 20/19-1 and OV 20/17-1)
© 2012 IUMS
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Reclassification and emended description of Caulobacter leidyi as Sphingomonas leidyi comb. nov., and emendation of the genus Sphingomonas
Int J Syst Evol Microbiol 62, 2835 (2012); https://doi.org/10.1099/ijs.0.039636-0
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