1887

Abstract

’ DSM 4733 has been shown to be affiliated with the family instead of the , and due to its poor characterization has been omitted from the current edition of and removed to limbo. We isolated a novel sphingoglycolipid-containing dimorphic prosthecate bacterium, designated strain 247, from a pre-alpine freshwater lake. Strain 247 and ‘ DSM 4733 were characterized in detail. The rod-shaped cells were Gram-stain-negative, aerobic, catalase- and oxidase-positive, and formed a stalk or polar flagellum. Both strains grew optimally at 28–30 °C, and pH 6.0–8.0. The major fatty acids were Cω7, C and 11-methyl Cω7. C 2-OH represents the major 2-hydroxy fatty acid. Q-10 was the major respiratory quinone and the major polar lipids were diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, three glycolipids, two phosphoaminolipids and two unidentified sphingoglycolipids. The major polyamine was -homospermidine. The G+C content of genomic DNA of strains 247 and DSM 4733 was 67.6 mol% and 67.0 mol%, respectively. According to 16S rRNA gene sequence analysis and DNA–DNA hybridization, strains DSM 4733 and 247 were phylogenetically closely related (99.6 % 16S rRNA gene sequence similarity, 82.9 % DNA–DNA hybridization value) and affiliated to the genus . The closest recognized species was DSM 15581 (98.1 % sequence similarity). In addition, the presence of cystine arylamidase, absence of β-galactosidase, and the inability to utilize -arabinose, galactose and sucrose distinguished strains DSM 4733 and 247 from most other members of the family . So far, the dimorphic life cycle that involves a prosthecate and a flagellated stage is unique for strains DSM 4733 and 247 among all members of the family . Therefore, is reclassified as , with the type strain DSM 4733 ( = ATCC 15260 = CIP 106443 = VKM B-1368) and strain 247 (DSM 25078 = LMG 26658) as an additional strain of this species.

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2012-12-01
2019-12-12
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