1887

Abstract

An aerobic, alkane-oxidizing bacterium, designated strain EPR92, was isolated from hydrothermal fluids that had been collected from a deep-sea vent on the East Pacific Rise (at 9° 50′ N 104° 17′ W). The cells of the novel strain were Gram-staining-negative rods that measured approximately 1.4 µm in length and 0.4 µm in width. Strain EPR92 grew at 20–40 °C (optimum 35 °C), with1.0–5.0 % (w/v) NaCl (optimum 2.5 %), and at pH 4.0–8.5 (optimum pH 7.5). The generation time under optimal conditions was 63 min. Strain EPR92 grew aerobically in artificial seawater minimal medium with -alkanes as sole carbon and energy sources, and also in artificial seawater medium supplemented with peptone and yeast extract. The predominant fatty acids were Cω7, C cyclo ω8, 11-methyl Cω7 and a putative C aldehyde. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and four unidentified aminolipids. The major respiratory quinone was Q-10 and the genomic DNA G+C content was 60.7 mol%. Phylogenetic analyses of the 16S rRNA gene showed that strain EPR92 belongs in the class and the recognized species that were most closely related to the novel strain were identified as P-31 (98.7 % sequence similarity) and DS-1 (95.8 %). In DNA–DNA hybridizations, the level of DNA–DNA relatedness observed between strain EPR92 and P-31 was 47.7 %, indicating that the two strains do not belong to the same species. Based on the phylogenetic, physiological, chemotaxonomic and genetic evidence, strain EPR92 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EPR92 ( = DSM 23209 = JCM 16666).

Funding
This study was supported by the:
  • National Science Foundation (Award ARRA OCE 09-37371, MCB 08-43678, MCB 04-56676 and OCE 03-27353)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.039594-0
2012-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/2921.html?itemId=/content/journal/ijsem/10.1099/ijs.0.039594-0&mimeType=html&fmt=ahah

References

  1. Berndt M. E., Allen D. E., Seyfried W. E. 1996; Reduction of CO2 during serpentinization of olivine at 300°C and 500 bar. Geology 24:351–354 [View Article]
    [Google Scholar]
  2. Brault M., Simoneit B. R. T., Marty J. C., Saliot A. 1988; Hydrocarbons in waters and particulate material from hydrothermal environments at the East Pacific Rise, 13°N. Org Geochem 12:209–219 [View Article]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  4. Crespo-Medina M., Chatziefthimiou A., Cruz-Matos R., Pérez-Rodríguez I., Barkay T., Lutz R. A., Starovoytov V., Vetriani C. 2009; Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera . Int J Syst Evol Microbiol 59:1497–1503 [View Article][PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  6. Gerhardt R., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Philips G. B. (eds). 1981; Manual of Methods for General Bacteriology . Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article]
    [Google Scholar]
  8. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  9. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [View Article]
    [Google Scholar]
  10. Lai Q., Wang L., Liu Y., Yuan J., Sun F., Shao Z. 2011; Parvibaculum indicum sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 61:271–274 [View Article][PubMed]
    [Google Scholar]
  11. Mattes T. E., Alexander A. K., Richardson P. M., Munk A. C., Han C. S., Stothard P., Coleman N. V. 2008; The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology. Appl Environ Microbiol 74:6405–6416 [View Article][PubMed]
    [Google Scholar]
  12. McCollom T. M., Ritter G., Simoneit B. R. 1999; Lipid synthesis under hydrothermal conditions by Fischer-Tropsch-type reactions. Orig Life Evol Biosph 29:153–166 [View Article][PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bact 39:159–167 [View Article]
    [Google Scholar]
  14. Pérez-Rodríguez I., Ricci J., Voordeckers J. W., Starovoytov V., Vetriani C. 2010; Nautilia nitratireducens sp. nov., a thermophilic, anaerobic, chemosynthetic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 60:1182–1186 [View Article][PubMed]
    [Google Scholar]
  15. Schleheck D., Tindall B. J., Rosselló-Mora R., Cook A. M. 2004; Parvibaculum lavamentivorans gen. nov., sp. nov., a novel heterotroph that initiates catabolism of linear alkylbenzenesulfonate. Int J Syst Evol Microbiol 54:1489–1497 [View Article][PubMed]
    [Google Scholar]
  16. Smits T. H. M., Röthlisberger M., Witholt B., van Beilen J. B. 1999; Molecular screening for alkane hydroxylase genes in Gram-negative and Gram-positive strains. Environ Microbiol 1:307–317 [View Article][PubMed]
    [Google Scholar]
  17. Tindall B. J. 1990a; A comparative-study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  18. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  19. van Beilen J. B., Funhoff E. G., van Loon A., Just A., Kaysser L., Bouza M., Holtackers R., Röthlisberger M., Li Z., Witholt B. 2006; Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases. Appl Environ Microbiol 72:59–65 [View Article][PubMed]
    [Google Scholar]
  20. Vetriani C., Speck M. D., Ellor S. V., Lutz R. A., Starovoytov V. 2004; Thermovibrio ammonificans sp. nov., a thermophilic, chemolithotrophic, nitrate-ammonifying bacterium from deep-sea hydrothermal vents. Int J Syst Evol Microbiol 54:175–181 [View Article][PubMed]
    [Google Scholar]
  21. Voordeckers J. W., Starovoytov V., Vetriani C. 2005; Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic, nitrate-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. Int J Syst Evol Microbiol 55:773–779 [View Article][PubMed]
    [Google Scholar]
  22. Wang B., Lai Q., Cui Z., Tan T., Shao Z. 2008; A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1. Environ Microbiol 10:1948–1963 [View Article][PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.039594-0
Loading
/content/journal/ijsem/10.1099/ijs.0.039594-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error