1887

Abstract

Two novel bacteria, designated strains Gsoil 634 and Dae 20, were isolated in South Korea from soil of a ginseng field and freshwater sediment, respectively and were characterized by a polyphasic approach to clarify their taxonomic positions. Phylogenetic analysis based on 16S rRNA gene sequences indicated that, although they probably represented two distinct species (indicated by a sequence similarity of 96.6 %), both strain Gsoil 634 and strain Dae 20 belonged to the genus and were most closely related to ‘ PB323 (97.8 % and 96.7 % sequence similarity, respectively), PB56 (96.8 % and 96.7 %), TDMA-17 (96.6 % and 95.4 %) and TDMA-16 (95.6 % and 95.8 %). For both novel strains, the major ubiquinone was Q-10, the major polyamine was homospermidine, the major cellular fatty acids included summed feature 7 (Cω7, Cω9 and/or Cω12), Cω6 and C, and the polar lipids included sphingoglycolipid. These chemotaxonomic data supported the affiliation of both strains to the genus . However, the DNA–DNA relatedness value between strain Gsoil 634 and PB323 was 31 %. Moreover, the results of physiological and biochemical tests allowed the phenotypic differentiation of strains Gsoil 634 and Dae 20 from established members of the genus . Based on these data, the two isolates represent two novel species in the genus , for which the names sp. nov. (type strain Gsoil 634 = KCTC 12630 = DSM 18094 = LMG 23739) and sp. nov. (type strain Dae 20  = KCTC 12629 = DSM 18106 = LMG 23592) are proposed.

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2013-02-01
2020-02-26
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