1887

Abstract

A Gram-positive, oxidase- and catalase-positive, strictly aerobic and motile bacterium, designated WS 4626, was isolated from a medical practice. Spherical endospores were formed terminally in swollen rods. The genomic DNA G+C content was 37.1 mol%. Cells contained iso-C, anteiso-C, iso-Cω10, anteiso-C and iso-C as the predominant cellular fatty acids and MK-7 and MK-6 as the major isoprenoid quinones. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, the cell-wall peptidoglycan was type A4α, -Lys--Asp and the major cell-wall sugar was xylose. The closest phylogenetic relatives were XDB9 (96.7 % 16S rRNA gene sequence similarity) and 34hs-1 (96.5 %). DNA–DNA relatedness between the isolate and DSM 18869 was very low (6 %). On the basis of the data presented, strain WS 4626 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WS 4626 ( = DSM 25057 = LMG 26643).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.039420-0
2013-04-01
2024-10-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/4/1512.html?itemId=/content/journal/ijsem/10.1099/ijs.0.039420-0&mimeType=html&fmt=ahah

References

  1. Ahmed I., Yokota A., Yamazoe A., Fujiwara T. ( 2007 ). Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.. Int J Syst Evol Microbiol 57, 11171125. [View Article] [PubMed]
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. ( 1991 ). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13, 202206. [View Article]
    [Google Scholar]
  3. Cerny G. ( 1976 ). Method for distinction of the Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol 3, 223225. [View Article]
    [Google Scholar]
  4. Coorevits A., Dinsdale A. E., Heyrman J., Schumann P., Van Landschoot A., Logan N. A., De Vos P. ( 2012 ). Lysinibacillus macroides sp. nov., nom. rev.. Int J Syst Evol Microbiol 62, 11211127. [View Article] [PubMed]
    [Google Scholar]
  5. Euzéby J. P. ( 2011 ). List of Prokaryotic names with Standing in Nomenclature. . http://www.bacterio.cict.fr/index.html
  6. Glazunova O. O., Raoult D., Roux V. ( 2006 ). Bacillus massiliensis sp. nov., isolated from cerebrospinal fluid. . Int J Syst Evol Microbiol 56, 14851488. [View Article] [PubMed]
    [Google Scholar]
  7. Gottheil O. ( 1901 ). Botanische Beschreibung einiger Bodenbakterien. . Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 7, 680691.
    [Google Scholar]
  8. Jung M. Y., Kim J.-S., Paek W. K., Styrak I., Park I.-S., Sin Y., Paek J., Park K. A., Kim H. & other authors ( 2012 ). Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus. . Int J Syst Evol Microbiol 62, 23472355. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  10. La Duc M. T., Satomi M., Venkateswaran K. ( 2004 ). Bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft. . Int J Syst Evol Microbiol 54, 195201. [View Article] [PubMed]
    [Google Scholar]
  11. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 29472948. [View Article] [PubMed]
    [Google Scholar]
  12. Lee C. S., Jung Y.-T., Park S., Oh T.-K., Yoon J.-H. ( 2010 ). Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus. . Int J Syst Evol Microbiol 60, 281286. [View Article] [PubMed]
    [Google Scholar]
  13. Logan N. A., De Vos P. ( 2009 ). Genus I. Bacillus . . In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 21128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  14. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  15. MacKenzie S. L. ( 1987 ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Off Anal Chem 70, 151160.[PubMed]
    [Google Scholar]
  16. Miwa H., Ahmed I., Yokota A., Fujiwara T. ( 2009 ). Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil. . Int J Syst Evol Microbiol 59, 14271432. [View Article] [PubMed]
    [Google Scholar]
  17. Neide E. ( 1904 ). Botanische Beschreibung einiger sporenbildender Bakterien. . Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 12, 337352.
    [Google Scholar]
  18. Ryu E. ( 1940 ). A simple method of differentiation between Gram-positive and Gram-negative organisms without staining. . Kitasato Arch Exp Med 17, 5863.
    [Google Scholar]
  19. Schmidt V. S., Mayr R., Wenning M., Glöckner J., Busse H.-J., Scherer S. ( 2009 ). Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese. . Int J Syst Evol Microbiol 59, 24372443. [View Article] [PubMed]
    [Google Scholar]
  20. Seiler H., Bleicher A., Busse H.-J., Hüfner J., Scherer S. ( 2012a ). Psychroflexus halocasei sp. nov., isolated from a aberrant cheese surface ripening microbial consortium. . Int J Syst Evol Microbiol 62, 18501856. [View Article] [PubMed]
    [Google Scholar]
  21. Seiler H., Schmidt V., Wenning M., Scherer S. ( 2012b ). Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. . Int J Syst Evol Microbiol 62, 10921097. [View Article] [PubMed]
    [Google Scholar]
  22. Shivaji S., Chaturvedi P., Begum Z., Pindi P. K., Manorama R., Padmanaban D. A., Shouche Y. S., Pawar S., Vaishampayan P. & other authors ( 2009 ). Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere. . Int J Syst Evol Microbiol 59, 29772986. [View Article] [PubMed]
    [Google Scholar]
  23. Smith N. R., Gordon R. E., Clark F. E. ( 1946 ). Aerobic Mesophilic Sporeforming Bacteria, misc. pub. no. 559. . Washington, DC:: USDA.
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  26. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60, 249266. [View Article] [PubMed]
    [Google Scholar]
  27. Wenning M., Scherer S., Naumann D. ( 2008 ). Infrared spectroscopy in the identification of microorganisms. . In Vibrational Spectroscopy for Medical Diagnosis, pp. 7196. Edited by Diem M., Griffith P. R., Chalmers J. M. . Chichester:: Wiley;.
    [Google Scholar]
  28. Whiton R. S., Lau P., Morgan S. L., Gilbart J., Fox A. ( 1985 ). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. . J Chromatogr A 347, 109120. [View Article] [PubMed]
    [Google Scholar]
  29. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [View Article] [PubMed]
    [Google Scholar]
  30. Yang L.-L., Huang Y., Liu J., Ma L., Mo M.-H., Li W.-J., Yang F.-X. ( 2012 ). Lysinibacillus mangiferahumi sp. nov., a new bacterium producing nematicidal volatiles. . Antonie van Leeuwenhoek 102, 5359. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.039420-0
Loading
/content/journal/ijsem/10.1099/ijs.0.039420-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error