1887

Abstract

Five strains of a previously uncharacterized anamorphic, methanol-assimilating yeast species are described here, for which the name is proposed. The strains were isolated from fruit waste collected in markets in Brunei, Borneo. The sequences of the D1/D2 domains of the large subunit rRNA genes, the internal transcribed spacer (ITS) regions and the 18S rRNA genes were identical between the isolates and differed from the corresponding sequences of all previously described yeast species. Phylogenetic analysis of these sequences showed that the new species formed a cluster with species of the genus , the closest related species being (6 % nucleotide substitutions in the D1/D2 domain). The type strain, 11-487, has been deposited in the Centralbureau voor Schimmelcultures (Utrecht, The Netherlands) as CBS 12507, the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 29-182-1 and the National Collection of Agricultural and Industrial Microorganisms (Budapest, Hungary) as NCAIM Y.02008. Mycobank no. MB563710.

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2012-09-01
2019-10-14
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References

  1. Bhadra B., Begum Z., Shivaji S.. ( 2008;). Pichia garciniae sp. nov., isolated from a rotten mangosteen fruit (Garcinia mangostana L., Clusiaceae). . Int J Syst Evol Microbiol 58:, 2665–2669. [CrossRef][PubMed]
    [Google Scholar]
  2. Felsenstein J.. ( 2007;). phylip (pylogeny inference package), version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  3. Fleet H. G.. ( 2003;). Yeasts in fruits and fruit products. . In Yeasts in Food, pp. 267–288. Edited by Boekhout T., Robert V... Hamburg:: Behr’s Verlag;. [CrossRef]
    [Google Scholar]
  4. Katoh K., Toh H.. ( 2008;). Recent developments in the mafft multiple sequence alignment program. . Brief Bioinform 9:, 286–298. [CrossRef][PubMed]
    [Google Scholar]
  5. Kurtzman C. P., Droby S.. ( 2001;). Metschnikowia fructicola, a new ascosporic yeast with potential for biocontrol of postharvest fruit rots. . Syst Appl Microbiol 24:, 395–399. [CrossRef][PubMed]
    [Google Scholar]
  6. Kurtzman C. P., Robnett C. J.. ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73:, 331–371. [CrossRef][PubMed]
    [Google Scholar]
  7. Kurtzman C. P., Robnett C. J.. ( 2010;). Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. . FEMS Yeast Res 10:, 353–361. [CrossRef][PubMed]
    [Google Scholar]
  8. Limtong S., Jindamorakot S., Am-In S., Kaewwichian R., Nitiyon S., Yongmanitchai W., Nakase T.. ( 2011;). Candida uthaithanina sp. nov., an anamorphic yeast species in Nakaseomyces clade isolated in Thailand. . Antonie van Leeuwenhoek 99:, 865–871. [CrossRef][PubMed]
    [Google Scholar]
  9. Nilsson R. H., Kristansson E., Ryberg M., Hallenberg N., Larsson K.-H.. ( 2008;). Intraspecific ITS variability in the Kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. . Evol Bioinform online 4:, 193–201.[PubMed]
    [Google Scholar]
  10. O’Donnell K.. ( 1993;). Fusarium and its near relatives. . In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 225–233. Edited by Reynolds D. R., Taylor J. W... Wallingford, UK:: CAB International;.
    [Google Scholar]
  11. Page R. D. M.. ( 1996;). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12:, 357–358.[PubMed]
    [Google Scholar]
  12. Péter G., Tornai-Lehoczki J., Suzuki M., Dlauchy D.. ( 2005;). Metschnikowia viticola sp. nov., a new yeast species from grape. . Antonie van Leeuwenhoek 87:, 155–160. [CrossRef][PubMed]
    [Google Scholar]
  13. Sipiczki M.. ( 2003;). Candida zemplinina sp. nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines. . Int J Syst Evol Microbiol 53:, 2079–2083. [CrossRef][PubMed]
    [Google Scholar]
  14. Sipiczki M.. ( 2006;). Metschnikowia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion. . Appl Environ Microbiol 72:, 6716–6724. [CrossRef][PubMed]
    [Google Scholar]
  15. Sipiczki M.. ( 2011;). Dimorphic cycle in Candida citri sp. nov., a novel yeast species isolated from rotting fruit in Borneo. . FEMS Yeast Res 11:, 202–208. [CrossRef][PubMed]
    [Google Scholar]
  16. Sipiczki M., Kajdacsi E.. ( 2009;). Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. . Int J Syst Evol Microbiol 59:, 914–920. [CrossRef][PubMed]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  18. van der Walt J. P., Yarrow D.. ( 1984;). Methods for the isolation, maintenance, classification and identification of yeasts. . In The Yeasts: a Taxonomic Study, pp. 45–104. Edited by Kreger-van Rij N. J. W... Amsterdam:: Elsevier;.
    [Google Scholar]
  19. White T. J., Burns T., Lee S., Taylor J.. ( 1990;). Amplification and sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols. A Guide to Methods and Applications, pp. 315–322. Edited by Innis M. A., Gelfand D. H., Snisky J. J., White T. J... San Diego, CA:: Acadamic Press;.
    [Google Scholar]
  20. Xue M.-L., Zhang L.-Q., Wang Q.-M., Zhang J.-S., Bai F.-Y.. ( 2006;). Metschnikowia sinensis sp. nov., Metschnikowia zizyphicola sp. nov. and Metschnikowia shanxiensis sp. nov., novel yeast species from jujube fruit. . Int J Syst Evol Microbiol 56:, 2245–2250. [CrossRef][PubMed]
    [Google Scholar]
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