sp. nov., isolated from a hexachlorocyclohexane dump site Free

Abstract

A yellow-pigmented bacterial strain, designated LL01, was isolated from hexachlorocyclohexane (HCH)-contaminated soil at Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01 occupied a distinct phylogenetic position in the cluster, showing highest similarity to CC-FH12-1 (98.5 %). The DNA G+C content of strain LL01 was 66.1 mol%. The predominant respiratory pigment was ubiquinone Q-10. The polar lipid profile of strain LL01 also corresponded to those reported for other species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as a member of the genus Spermidine was the major polyamine observed. The results obtained from DNA–DNA hybridization and biochemical and physiological tests clearly distinguished strain LL01 from closely related species of the genus . Therefore, strain LL01 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain LL01 = CCM 7979 = DSM 25410).

Funding
This study was supported by the:
  • Department of Biotechnology (DBT)
  • National Bureau of Agriculturally Important Microorganisms (NBAIM, ICAR)
  • DU/DST-PURSE grant, Government of India
  • Grant Agency of the Czech Republic (Award P202/10/1435 and 203/08/0114)
  • Grant Agency of the Czech Academy of Sciences (Award IAA401630901)
  • European Regional Development Fund (Award CZ.1.05/1.1.00/02.0123 and CZ.1.05/2.1.00/01.0001)
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2013-02-01
2024-03-30
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