A yellow-pigmented bacterial strain, designated LL01T, was isolated from hexachlorocyclohexane (HCH)-contaminated soil at Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01T occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity to Sphingobium rhizovicinum CC-FH12-1T (98.5 %). The DNA G+C content of strain LL01T was 66.1 mol%. The predominant respiratory pigment was ubiquinone Q-10. The polar lipid profile of strain LL01T also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as a member of the genus Sphingobium. Spermidine was the major polyamine observed. The results obtained from DNA–DNA hybridization and biochemical and physiological tests clearly distinguished strain LL01T from closely related species of the genus Sphingobium. Therefore, strain LL01T represents a novel species of the genus Sphingobium, for which the name Sphingobium czechense sp. nov. is proposed (type strain LL01T = CCM 7979T = DSM 25410T).
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Funding
This study was supported by the:
Department of Biotechnology (DBT)
National Bureau of Agriculturally Important Microorganisms (NBAIM, ICAR)
DU/DST-PURSE grant, Government of India
Grant Agency of the Czech Republic
(Award P202/10/1435 and 203/08/0114)
Grant Agency of the Czech Academy of Sciences
(Award IAA401630901)
European Regional Development Fund
(Award CZ.1.05/1.1.00/02.0123 and CZ.1.05/2.1.00/01.0001)
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