1887

Abstract

A Gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated A1, was isolated from sludge of a wastewater treatment plant. Strain A1 showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. Strain A1 grew with 0–11 % (w/v) NaCl, at 20–42 °C, but not <15 or >45 °C and at pH 6–10 (optimum pH 8.0–9.0). The isolate was catalase-positive and oxidase-negative. The DNA G+C content was 70.7 mol%. A comparative analysis of 16S rRNA gene sequences revealed that strain A1 formed a distinct phyletic lineage in the genus and showed high sequence similarity with NCDO 2048 (96.9 %), KMM 3637 (96.9 %) and CF63 (96.4 %). DNA–DNA hybridization revealed <43 % DNA–DNA relatedness between the isolate and its closest phylogenetic relatives. The affiliation of strain A1 with the genus was supported by the chemotaxonomic data: predominant quinone menaquinone MK-7(H); polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid -diaminopimelic acid; whole-cell sugars galactose, xylose and ribose; absence of mycolic acids; and major fatty acids iso-C, anteiso-C and anteiso-C. The results of physiological and biochemical tests allowed phenotypic differentiation of strain A1 from members of the genus . On the basis of the results in this study, a novel species, sp. nov., is proposed. The type strain is A1 ( = KEMC 41-098  = JCM 17537  = KACC 15558).

Funding
This study was supported by the:
  • , Korea Ministry of Educational Science and Technology , (Award 2011-0000544)
  • , Korea National Environmental Micro-organisms Bank (KEMB)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.039305-0
2013-03-01
2020-08-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/1111.html?itemId=/content/journal/ijsem/10.1099/ijs.0.039305-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. ( 1993 ). Handbook of Microbiological Media. Edited by L.C. Parks. Boca Raton, FL: CRC Press. . [CrossRef]
    [Google Scholar]
  2. Bhadra B., Raghukumar C., Pindi P. K., Shivaji S. ( 2008 ). Brevibacterium oceani sp. nov., isolated from deep-sea sediment of the Chagos Trench, Indian Ocean. . Int J Syst Evol Microbiol 58, 5760. [CrossRef] [PubMed]
    [Google Scholar]
  3. Breed R. S. ( 1953 ). The Brevibacteriaceae fam. nov. of order Eubacteriales. Riass Commun VI Congr Int Microbiol Roma 1, 13–14.
  4. Brown A. E. ( 2008 ). Benson’s Microbiological Applications: Laboratory Manual in General Microbiology, , 10th edn.. Boston, MA:: McGraw-Hill, Inc;.
    [Google Scholar]
  5. Cai J., Collins M. D. ( 1994 ). Phylogenetic analysis of species of the meso-diaminopimelic acid-containing genera Brevibacterium and Dermabacter . . Int J Syst Bacteriol 44, 583585. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cappuccino J. G., Sherman N. ( 2010 ). Microbiology: A Laboratory Manual, , 9th edn.. San Francisco:: Benjamin Cummings;. pp. 6974 & 161164.
    [Google Scholar]
  7. Collins M. D. ( 2006 ). The Genus Brevibacterium . . In Prokaryotes, vol. 3, pp. 10131019. Edited by Dworkin M, Falkow S, Rosenberg E, Schleifer K.-H, Stackebrandt E. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Collins M. D., Goodfellow M., Minnikin D. E. ( 1979 ). Isoprenoid quinones in the classification of coryneform and related bacteria. . J Gen Microbiol 110, 127136. [CrossRef] [PubMed]
    [Google Scholar]
  9. Collins M. D., Jones D., Keddie R. M., Sneath P. H. A. ( 1980 ). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev.; comb. nov.. J Gen Microbiol 120, 110.
    [Google Scholar]
  10. Collins M. D., Farrow J. A. E., Goodfellow M., Minnikin D. E. ( 1983 ). Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov.. Syst Appl Microbiol 4, 388395. [CrossRef]
    [Google Scholar]
  11. DSMZ ( 2001 ). Catalogue of Strains, , 7th edn.. Braunschweig:: Deutsche Sammlung von Mikroorganismen und Zellkulturen;.
    [Google Scholar]
  12. Ezaki T., Suzuki S. ( 1982 ). Achromopeptidase for lysis of anaerobic gram-positive cocci. . J Clin Microbiol 16, 844846.[PubMed]
    [Google Scholar]
  13. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [CrossRef]
    [Google Scholar]
  14. Felsenstein J. ( 1985 ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  15. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  16. Frank J. A., Reich C. I., Sharma S., Weisbaum J. S., Wilson B. A., Olsen G. J. ( 2008 ). Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. . Appl Environ Microbiol 74, 24612470. [CrossRef] [PubMed]
    [Google Scholar]
  17. Gavrish E. Iu., Krauzova V. I., Potekhina N. V., Karasev S. G., Plotnikova E. G., Altyntseva O. V., Korosteleva L. A., Evtushenko L. I. ( 2004 ). [Three new species of brevibacteria – Brevibacterium antiquum sp. nov., Brevibacterium aurantiacum sp. nov. and Brevibacterium permense sp. nov.]. Mikrobiologiia 73, 218225 (in Russian).[PubMed]
    [Google Scholar]
  18. Gruner E., Pfyffer G. E., von Graevenitz A. ( 1993 ). Characterization of Brevibacterium spp. from clinical specimens. . J Clin Microbiol 31, 14081412.[PubMed]
    [Google Scholar]
  19. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  20. Hayat M. A., Miller S. E. ( 1990 ). Negative Staining: Applications and Methods. New York:: McGraw-Hill Publishing Co;.
    [Google Scholar]
  21. Heyrman J., Verbeeren J., Schumann P., Devos J., Swings J., De Vos P. ( 2004 ). Brevibacterium picturae sp. nov., isolated from a damaged mural painting at the Saint-Catherine chapel (Castle Herberstein, Austria). . Int J Syst Evol Microbiol 54, 15371541. [CrossRef] [PubMed]
    [Google Scholar]
  22. Hiraishi A., Ueda Y., Ishihara J., Mori T. ( 1996 ). Comparative lipoquinone analysis of influent sewage and activated sludge by high performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42, 457469. [CrossRef]
    [Google Scholar]
  23. Ivanova E. P., Christen R., Alexeeva Y. V., Zhukova N. V., Gorshkova N. M., Lysenko A. M., Mikhailov V. V., Nicolau D. V. ( 2004 ). Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga. . Int J Syst Evol Microbiol 54, 21072111. [CrossRef] [PubMed]
    [Google Scholar]
  24. Jones D., Keddie R. M. ( 1986 ). Genus Brevibacterium . . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 13011313. Edited by Sneath P. H. A., Mair N., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  25. Kämpfer P., Schäfer J., Lodders N., Busse H.-J. ( 2010 ). Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment. . Int J Syst Evol Microbiol 60, 909913. [CrossRef] [PubMed]
    [Google Scholar]
  26. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  27. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [CrossRef]
    [Google Scholar]
  28. Lee S. D. ( 2008 ). Brevibacterium marinum sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58, 500504. [CrossRef] [PubMed]
    [Google Scholar]
  29. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  30. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  31. Minnikin D. E., Alshamaony L., Goodfellow M. ( 1975 ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88, 200204. [CrossRef]
    [Google Scholar]
  32. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [CrossRef]
    [Google Scholar]
  33. Pascual C., Collins M. D. ( 1999 ). Brevibacterium avium sp. nov., isolated from poultry. . Int J Syst Bacteriol 49, 15271530. [CrossRef] [PubMed]
    [Google Scholar]
  34. Roux V., Raoult D. ( 2009 ). Brevibacterium massiliense sp. nov., isolated from a human ankle discharge. . Int J Syst Evol Microbiol 59, 19601964. [CrossRef] [PubMed]
    [Google Scholar]
  35. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  36. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  37. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  38. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [CrossRef]
    [Google Scholar]
  39. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  40. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  41. Wauters G., Haase G., Avesani V., Charlier J., Janssens M., Van Broeck J., Delmée M. ( 2004 ). Identification of a novel Brevibacterium species isolated from humans and description of Brevibacterium sanguinis sp. nov.. J Clin Microbiol 42, 28292832. [CrossRef] [PubMed]
    [Google Scholar]
  42. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.039305-0
Loading
/content/journal/ijsem/10.1099/ijs.0.039305-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error