1887

Abstract

A Gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated A1, was isolated from sludge of a wastewater treatment plant. Strain A1 showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. Strain A1 grew with 0–11 % (w/v) NaCl, at 20–42 °C, but not <15 or >45 °C and at pH 6–10 (optimum pH 8.0–9.0). The isolate was catalase-positive and oxidase-negative. The DNA G+C content was 70.7 mol%. A comparative analysis of 16S rRNA gene sequences revealed that strain A1 formed a distinct phyletic lineage in the genus and showed high sequence similarity with NCDO 2048 (96.9 %), KMM 3637 (96.9 %) and CF63 (96.4 %). DNA–DNA hybridization revealed <43 % DNA–DNA relatedness between the isolate and its closest phylogenetic relatives. The affiliation of strain A1 with the genus was supported by the chemotaxonomic data: predominant quinone menaquinone MK-7(H); polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid -diaminopimelic acid; whole-cell sugars galactose, xylose and ribose; absence of mycolic acids; and major fatty acids iso-C, anteiso-C and anteiso-C. The results of physiological and biochemical tests allowed phenotypic differentiation of strain A1 from members of the genus . On the basis of the results in this study, a novel species, sp. nov., is proposed. The type strain is A1 ( = KEMC 41-098  = JCM 17537  = KACC 15558).

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2013-03-01
2019-10-22
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