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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_1

sp. nov. and sp. nov., isolated from sea sediment Free

Abstract

Two novel Gram-stain-positive bacteria, designated HR08-7 and HR08-43, were isolated from a sea sediment sample from Rishiri Island, Hokkaido, Japan, and their taxonomic positions were investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strains HR08-7 and HR08-43 and the members of the genus formed a monophyletic cluster with similarity range of 95.5–99.0 %. The peptidoglycan type of strains HR08-7 and HR08-43 was A4β. The predominant menaquinone of both strains was demethylmenaquinone DMK-9(H) and the major fatty acid was anteiso-C. The DNA G+C contents of strains HR08-7 and HR08-43 were 64.5 and 62.4 mol%, respectively. The results of phylogenetic analysis and DNA–DNA hybridization, along with differences of strains HR08-7 and HR08-43 from the recognized species in phenotypic characteristics, indicate that the two strains merit classification as representatives of two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed; the type strains are HR08-7 ( = NBRC 105854 = DSM 24865) and HR08-43 ( = NBRC 105855 = DSM 24867), respectively.

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Funding
This study was supported by the:
  • Institute for Fermentation, Osaka (IFO)
© 2013 IUMS
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2013-01-01
2025-03-24
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Demequinaflava sp. nov. and Demequinasediminicola sp. nov., isolated from sea sediment
Int J Syst Evol Microbiol 63, 249 (2013); https://doi.org/10.1099/ijs.0.039297-0
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