RT Journal Article SR Electronic(1) A1 Kong, Bi He A1 Li, Yan Hong A1 Liu, Min A1 Liu, Yang A1 Li, Chun Li A1 Liu, Lei A1 Yang, Zhi Wei A1 Yu, RongYR 2013 T1 Massilia namucuonensis sp. nov., isolated from a soil sample JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_1 SP 352 OP 357 DO https://doi.org/10.1099/ijs.0.039255-0 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain 333-1-0411T, was isolated from a soil sample collected from Namucuo, Tibet Autonomous Region, China and characterized in a taxonomic study using a polyphasic approach. The major fatty acid components of strain 333-1-0411T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0; its major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Q-8 was the dominant ubiquinone, and the G+C content of the genomic DNA was 66.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 333-1-0411T fell within the evolutionary radiation encompassed by the genus Massilia . The 16S rRNA gene sequence similarities between strain 333-1-0411T and recognized species of the genus Massilia ranged from 95.4 % to 97.2 %, and the most closely related strains were Massilia flava Y9T (97.2 %) and Massilia albidiflava 45T (97.0 %). However, the DNA–DNA relatedness values between strain 333-1-0411T and M. flava Y9T and M. albidiflava 45T were 20.2 % and 27.2 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 333-1-0411T is considered to represent a novel species of the genus Massilia , for which the name Massilia namucuonensis sp. nov. is proposed. The type strain is 333-1-0411T ( = CGMCC 1.11014T = DSM 25159T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.039255-0