1887

Abstract

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain 333-1-0411, was isolated from a soil sample collected from Namucuo, Tibet Autonomous Region, China and characterized in a taxonomic study using a polyphasic approach. The major fatty acid components of strain 333-1-0411 were summed feature 3 (Cω7 and/or Cω6) and C; its major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Q-8 was the dominant ubiquinone, and the G+C content of the genomic DNA was 66.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 333-1-0411 fell within the evolutionary radiation encompassed by the genus . The 16S rRNA gene sequence similarities between strain 333-1-0411 and recognized species of the genus ranged from 95.4 % to 97.2 %, and the most closely related strains were Y9 (97.2 %) and 45 (97.0 %). However, the DNA–DNA relatedness values between strain 333-1-0411 and Y9 and 45 were 20.2 % and 27.2 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 333-1-0411 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 333-1-0411 ( = CGMCC 1.11014 = DSM 25159).

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2013-01-01
2019-12-06
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References

  1. Collins M. D. , Howarth O. W. , Grund E. , Kroppenstedt R. M. . ( 1987; ). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis . . FEMS Microbiol Lett 41:, 35–39. [CrossRef]
    [Google Scholar]
  2. Dong X.-Z. , Cai M.-Y. . ( 2001; ). [ Determination of biochemical properties]. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Edited by Dong X.-Z. , Cai M.-Y. . . Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  3. Gallego V. , Sánchez-Porro C. , García M. T. , Ventosa A. . ( 2006; ). Massilia aurea sp. nov., isolated from drinking water. . Int J Syst Evol Microbiol 56:, 2449–2453. [CrossRef] [PubMed]
    [Google Scholar]
  4. Gillis M. , De Ley J. , De Cleene M. . ( 1970; ). The determination of molecular weight of bacterial genome DNA from renaturation rates. . Eur J Biochem 12:, 143–153. [CrossRef] [PubMed]
    [Google Scholar]
  5. Kämpfer P. , Falsen E. , Busse H.-J. . ( 2008; ). Naxibacter varians sp. nov. and Naxibacter haematophilus sp. nov., and emended description of the genus Naxibacter . . Int J Syst Evol Microbiol 58:, 1680–1684. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kämpfer P. , Lodders N. , Martin K. , Falsen E. . ( 2011; ). Revision of the genus Massilia La Scola et al. 2000, with an emended description of the genus and inclusion of all species of the genus Naxibacter as new combinations, and proposal of Massilia consociata sp. nov.. Int J Syst Evol Microbiol 61:, 1528–1533. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , Yi H. , Won S. , Chun J. . ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721.[PubMed] [CrossRef]
    [Google Scholar]
  8. La Scola B. , Birtles R. J. , Mallet M. N. , Raoult D. . ( 1998; ). Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. . J Clin Microbiol 36:, 2847–2852.[PubMed]
    [Google Scholar]
  9. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  10. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  11. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  12. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  13. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  14. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  15. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  17. Wang J. , Zhang J. , Pang H. , Zhang Y. , Li Y. , Fan J. . ( 2012; ). Massilia flava sp. nov., isolated from soil. . Int J Syst Evol Microbiol 62:, 580–585. [CrossRef] [PubMed]
    [Google Scholar]
  18. Weon H.-Y. , Kim B.-Y. , Son J.-A. , Jang H. B. , Hong S. K. , Go S.-J. , Kwon S.-W. . ( 2008; ). Massilia aerilata sp. nov., isolated from an air sample. . Int J Syst Evol Microbiol 58:, 1422–1425. [CrossRef] [PubMed]
    [Google Scholar]
  19. Weon H.-Y. , Kim B.-Y. , Hong S.-B. , Jeon Y.-A. , Koo B.-S. , Kwon S.-W. , Stackebrandt E. . ( 2009; ). Massilia niabensis sp. nov. and Massilia niastensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 59:, 1656–1660. [CrossRef] [PubMed]
    [Google Scholar]
  20. Weon H.-Y. , Yoo S.-H. , Kim S.-J. , Kim Y.-S. , Anandham R. , Kwon S.-W. . ( 2010; ). Massilia jejuensis sp. nov. and Naxibacter suwonensis sp. nov., isolated from air samples. . Int J Syst Evol Microbiol 60:, 1938–1943. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  22. Xu P. , Li W. J. , Tang S. K. , Zhang Y. Q. , Chen G. Z. , Chen H. H. , Xu L. H. , Jiang C. L. . ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef] [PubMed]
    [Google Scholar]
  23. Zhang Y. Q. , Li W. J. , Zhang K. Y. , Tian X. P. , Jiang Y. , Xu L. H. , Jiang C. L. , Lai R. . ( 2006; ). Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. . Int J Syst Evol Microbiol 56:, 459–463. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zul D. , Wanner G. , Overmann J. . ( 2008; ). Massilia brevitalea sp. nov., a novel betaproteobacterium isolated from lysimeter soil. . Int J Syst Evol Microbiol 58:, 1245–1251. [CrossRef] [PubMed]
    [Google Scholar]
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