1887

Abstract

A facultatively anaerobic, alkaliphilic, Gram-stain-positive, rod-shaped bacterium, designated strain 12/1, isolated from alkaline wastewater drained sludge of a beverage industry facility located near New Delhi, India, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain 12/1 belonged to the genus and was most closely related to DSM 6208 (99.46 %), IMTB-3094 (99.18 %), 8N (99.06 %), 10C (98.17 %), TF-16 (98.1 %) and TF-80 (98.03 %). The DNA G+C content of strain 12/1 was 55.6 mol%, major respiratory isoprenoid quinone was MK-7, major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine and the cell-wall peptidoglycan was of the A3α -Lys–Gly type, characteristics consistent with its affiliation to the genus . Strain 12/1 showed levels of DNA–DNA hybridization of less than 70 % with the closely related species of the genus . Overall, the phenotypic, chemotaxonomic and phylogenetic data presented in this study suggest that strain 12/1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 12/1 ( = CCM 8459 = DSM 21148).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.039123-0
2013-12-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/12/4374.html?itemId=/content/journal/ijsem/10.1099/ijs.0.039123-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 2005;). Handbook of Media for Environmental Microbiology, , 2nd edn.. Boca Raton, FL:: CRC Press/ Taylor & Francis Group;. [CrossRef]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J.. ( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37:, 911–917. [CrossRef][PubMed]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  4. Chaturvedi P., Shivaji S.. ( 2006;). Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. . Int J Syst Evol Microbiol 56:, 2765–2770. [CrossRef][PubMed]
    [Google Scholar]
  5. Chaturvedi P., Prabahar V., Manorama R., Pindi P. K., Bhadra B., Begum Z., Shivaji S.. ( 2008;). Exiguobacterium soli sp. nov., a psychrophilic bacterium from the McMurdo Dry Valleys, Antarctica. . Int J Syst Evol Microbiol 58:, 2447–2453. [CrossRef][PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  7. Collins M. D., Lund B. M., Farrow J. A. E., Schleifer K.. ( 1983;). Chemotaxonomic study of an alkalophilic bacterium, Exiguobacterium aurantiacum gen. nov., sp. nov.. J Gen Microbiol 129:, 2037–2042.
    [Google Scholar]
  8. Crapart S., Fardeau M. L., Cayol J. L., Thomas P., Sery C., Ollivier B., Combet-Blanc Y.. ( 2007;). Exiguobacterium profundum sp. nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol 57:, 287–292. [CrossRef][PubMed]
    [Google Scholar]
  9. Farrow J. A. E., Wallbanks S., Collins M. D.. ( 1994;). Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera Caryophanon, Exiguobacterium, Kurthia, and Planococcus. . Int J Syst Bacteriol 44:, 74–82. [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: An approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Frühling A., Schumann P., Hippe H., Sträubler B., Stackebrandt E.. ( 2002;). Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov.. Int J Syst Evol Microbiol 52:, 1171–1176. [CrossRef][PubMed]
    [Google Scholar]
  12. Kim I. G., Lee M. H., Jung S. Y., Song J. J., Oh T. K., Yoon J. H.. ( 2005;). Exiguobacterium aestuarii sp. nov. and Exiguobacterium marinum sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 55:, 885–889. [CrossRef][PubMed]
    [Google Scholar]
  13. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  14. Kulshreshtha N. M., Kumar A., Dhall P., Gupta S., Bisht G., Pasha S., Singh V. P., Kumar R.. ( 2010;). Neutralization of alkaline industrial wastewaters using Exiguobacterium sp.. Int Biodeterior Biodegradation 64:, 191–196. [CrossRef]
    [Google Scholar]
  15. López-Cortés A., Schumann P., Pukall R., Stackebrandt E.. ( 2006;). Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana. . Syst Appl Microbiol 29:, 183–190. [CrossRef][PubMed]
    [Google Scholar]
  16. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  18. Prakash O., Kumari K., Lal R.. ( 2007;). Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. . Int J Syst Evol Microbiol 57:, 527–531. [CrossRef][PubMed]
    [Google Scholar]
  19. Raichand R., Pareek S., Singh N. K., Mayilraj S.. ( 2012;). Exiguobacterium aquaticum sp. nov., a member of the genus Exiguobacterium. . Int J Syst Evol Microbiol 62:, 2150–2155. [CrossRef][PubMed]
    [Google Scholar]
  20. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. ( 1955;). The behaviour of the isomers of 2,6-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  21. Rodrigues D. F., Goris J., Vishnivetskaya T., Gilichinsky D., Thomashow M. F., Tiedje J. M.. ( 2006;). Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov.. Extremophiles 10:, 285–294. [CrossRef][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sato N. S., Murata N.. ( 1988;). Membrane lipids. . In Methods in Enzymology, vol. 167, pp. 251–259. Edited by Packer L., Glazer A. N... NY:: Academic Press;.
    [Google Scholar]
  24. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  25. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  28. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  29. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  32. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  33. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  34. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M... Washington, DC, USA:: L. R. Snyder American Society for Microbiology;.
    [Google Scholar]
  35. Tourova T. P., Antonov A. S.. ( 1988;). Identification of microorganisms by rapid DNA-DNA hybridization. . Methods Microbiol 19:, 333–355. [CrossRef]
    [Google Scholar]
  36. Yumoto I., Hishinuma-Narisawa M., Hirota K., Shingyo T., Takebe F., Nodasaka Y., Matsuyama H., Hara I.. ( 2004;). Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity. . Int J Syst Evol Microbiol 54:, 2013–2017. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.039123-0
Loading
/content/journal/ijsem/10.1099/ijs.0.039123-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error