1887

Abstract

A facultatively anaerobic, alkaliphilic, Gram-stain-positive, rod-shaped bacterium, designated strain 12/1, isolated from alkaline wastewater drained sludge of a beverage industry facility located near New Delhi, India, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain 12/1 belonged to the genus and was most closely related to DSM 6208 (99.46 %), IMTB-3094 (99.18 %), 8N (99.06 %), 10C (98.17 %), TF-16 (98.1 %) and TF-80 (98.03 %). The DNA G+C content of strain 12/1 was 55.6 mol%, major respiratory isoprenoid quinone was MK-7, major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine and the cell-wall peptidoglycan was of the A3α -Lys–Gly type, characteristics consistent with its affiliation to the genus . Strain 12/1 showed levels of DNA–DNA hybridization of less than 70 % with the closely related species of the genus . Overall, the phenotypic, chemotaxonomic and phylogenetic data presented in this study suggest that strain 12/1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 12/1 ( = CCM 8459 = DSM 21148).

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2013-12-01
2020-08-11
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