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Abstract

A Gram-negative-staining, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, DPG-138, was isolated from seawater on the southern coast in Korea. Strain DPG-138 grew optimally at 30 °C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DPG-138 fell within the cluster comprising the type strains of four species of the genus (sequence similarity values of 97.8–98.2 %). The DNA G+C content was 59.9 mol%. The predominant ubiquinone of strain DPG-138 was Q-10, typical for the genus . The cellular fatty acid profiles of strain DPG-138 and type strains of four species of the genus were similar with Cω7 as the most common fatty acid. The major polar lipids in strain DPG-138 were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Differential phenotypic properties and genetic distinctiveness of strain DPG-138 demonstrated that this strain is distinguishable from all four recognized species of the genus . On the basis of the data presented, strain DPG-138 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DPG-138 ( = KCTC 23882 = CCUG 62112).

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • Ministry of Education, Science and Technology (MEST)
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2013-02-01
2021-10-21
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References

  1. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  3. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 2568. [View Article]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  6. Hwang C. Y., Bae G. D., Yih W., Cho B. C. ( 2009 ). Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats. . Int J Syst Evol Microbiol 59, 15681575. [View Article] [PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  8. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  9. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  10. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  11. Ostle A. G., Holt J. G. ( 1982 ). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. . Appl Environ Microbiol 44, 238241.[PubMed]
    [Google Scholar]
  12. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  13. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  14. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  15. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  17. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  18. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  19. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 5357. [View Article] [PubMed]
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2010 ). Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 60, 196199. [View Article] [PubMed]
    [Google Scholar]
  21. Yoon J.-H., Kang S.-J., Lee S.-Y., Jung Y.-T., Lee J.-S., Oh T.-K. ( 2012 ). Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. . Int J Syst Evol Microbiol 62, 839843. [View Article] [PubMed]
    [Google Scholar]
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