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Abstract

A Gram-staining negative, strictly aerobic bacterium, designated 101-1, was isolated from a sea tidal flat, Taean, Korea. The strain formed small light-yellow, smooth, and circular colonies on marine agar. Cells were weakly halophilic, motile rods showing catalase- and oxidase-positive reactions. Growth of strain 101-1 was observed at 15–40 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5–7.0) and 1.0–9.0 % (w/v) NaCl (optimum, 2.0–3.5 %). The G+C content of the genomic DNA was 53.3 mol%. Strain 101-1 contained ubiquinone-10 (Q-10) as the respiratory quinone and iso-Cω9, iso-C and iso-C as major fatty acids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 101-1 formed a tight phylogenetic lineage with members of the genus and was most closely related to GW14-5 and S3-22 with 97.3 % and 96.3 % 16S rRNA gene sequence similarities, respectively. The DNA–DNA relatedness values between strain 101-1 and GW14-5 and S3-22 were 24.8±4.4 % and 32.2±3.6 %, respectively. Based on the data from the phenotypic and genotypic studies, strain 101-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 101-1 ( = KACC 16184 = JCM 17742).

Funding
This study was supported by the:
  • , National Research Foundation of Korea , (Award No. 2010-0026359)
  • , Korea Government (MEST)
  • , Next-Generation BioGreen 21 Program , (Award No. SSAC2011- PJ008220)
  • , Rural Development Administration
  • , Young Investigator Research Program of Chung-Ang University Research Scholarship
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2012-12-01
2020-08-05
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