1887

Abstract

A novel actinomycete strain, PS7-2, which produced spore chains borne on the tips of short sporophores, was isolated from soil collected from a temperate peat swamp forest in Phu-Sang National Park, Phayao province, Thailand. The isolate contained glutamic acid, glycine, alanine, 3-hydroxy-diaminopimelic acid and -diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars were glucose, mannose, rhamnose and xylose, and the major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The predominant menaquinones were MK-10(H) and MK-9(H) and the predominant cellular fatty acids were iso-C, anteiso-C and anteiso-C. The G+C content of the genomic DNA was 72.3 mol%. On the basis of 16S rRNA gene sequence analysis, strain PS7-2 clustered with members of the genus and appeared most closely related to the type strains of (99.5 % sequence similarity), (99.0 %), (98.9 %) and (98.5 %). DNA–DNA hybridizations and some physiological and biochemical properties indicated that strain PS7-2 could be readily distinguished from its closest phylogenetic relatives. Based on the phenotypic and genotypic evidence and DNA–DNA relatedness values, strain PS7-2 represents a novel species in the genus , for which the name sp. nov. is proposed; the type strain is PS7-2 ( = BCC 41921 = NBRC 107932).

Funding
This study was supported by the:
  • , Thailand Graduate Institute of Science and Technology of the National Science and Technology Development Agency , (Award TG-22-22-52-021M)
  • , Department of Biology and Microbial Resource Management Unit of the Scientific Instrument Centre, Faculty of Science, King Mongkut’s Institute of Technology, Ladkrabang, Bangkok, Thailand
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2013-01-01
2020-07-03
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