1887

Abstract

Thirteen strains isolated from virgin olive oil or its by-products in several Mediterranean countries were found to be phenotypically and genetically divergent from currently recognized yeast species. Sequence analysis of the large subunit (LSU) rDNA D1/D2 domain and internal transcribed spacer regions/5.8S rDNA revealed that the strains represented two novel species described as sp. nov. (type strain ZIM 2334 = CBS 12504 = NCAIM Y.02001) and sp. nov. (type strain DBVPG 5508 = CBS 12508 = NCAIM Y.02000). Phylogenetic analysis based on concatenated sequences of the small subunit rRNA gene, the D1/D2 region of the LSU rDNA and the translation elongation factor-1α gene suggested that sp. nov. and sp. nov. should be placed within the and clades, respectively.

Funding
This study was supported by the:
  • Slovenian Research Agency (Award MRIC-UL ZIM, IP-0510 and P4-0116)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038794-0
2012-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2296.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038794-0&mimeType=html&fmt=ahah

References

  1. Bleve G., Lezzi C., Chiriatti M. A., D’Ostuni I., Tristezza M., Di Venere D., Sergio L., Mita G., Grieco F. 2011; Selection of non-conventional yeasts and their use in immobilized form for the bioremediation of olive oil mill wastewaters. Bioresour Technol 102:982–989 [View Article][PubMed]
    [Google Scholar]
  2. Cadez N., Poot G. A., Raspor P., Smith M. T. 2003; Hanseniaspora meyeri sp. nov., Hanseniaspora clermontiae sp. nov., Hanseniaspora lachancei sp. nov. and Hanseniaspora opuntiae sp. nov., novel apiculate yeast species. Int J Syst Evol Microbiol 53:1671–1680 [View Article][PubMed]
    [Google Scholar]
  3. Cadez N., Raspor P., Smith M. T. 2006; Phylogenetic placement of Hanseniaspora-Kloeckera species using multigene sequence analysis with taxonomic implications: descriptions of Hanseniaspora pseudoguilliermondii sp. nov. and Hanseniaspora occidentalis var. citrica var. nov.. Int J Syst Evol Microbiol 56:1157–1165 [View Article][PubMed]
    [Google Scholar]
  4. Ciafardini G., Zullo B. A. 2002; Microbiological activity in stored olive oil. Int J Food Microbiol 75:111–118 [View Article][PubMed]
    [Google Scholar]
  5. Ciafardini G., Zullo B. A., Iride A. 2006a; Lipase production by yeasts from extra virgin olive oil. Food Microbiol 23:60–67 [View Article][PubMed]
    [Google Scholar]
  6. Ciafardini G., Zullo B. A., Cioccia G., Iride A. 2006b; Lipolytic activity of Williopsis californica and Saccharomyces cerevisiae in extra virgin olive oil. Int J Food Microbiol 107:27–32 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Koidis A., Triantafillou E., Boskou D. 2008; Endogenous microflora in turbid virgin olive oils and the physicochemical characteristics of these oils. Eur J Lipid Sci Technol 110:164–171 [View Article]
    [Google Scholar]
  9. Kurtzman C. P. 2011a; Lindnera Kurtzman, Robnett & Basehoar-Powers (2008). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 pp. 521–543 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  10. Kurtzman C. P. 2011b; Nakazawaea Y. Yamada, Maeda & Mikata (1994). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 pp. 637–639 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  11. Kurtzman C. P. 2011c; Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus . Antonie van Leeuwenhoek 99:13–23 [View Article][PubMed]
    [Google Scholar]
  12. Kurtzman C. P., Robnett C. J. 2003; Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3:417–432 [View Article][PubMed]
    [Google Scholar]
  13. Kurtzman C. P., Robnett C. J. 2010; Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales . FEMS Yeast Res 10:353–361 [View Article][PubMed]
    [Google Scholar]
  14. Kurtzman C. P., Robnett C. J., Basehoar-Powers E. 2008; Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov.. FEMS Yeast Res 8:939–954 [View Article][PubMed]
    [Google Scholar]
  15. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, a Taxonomic Study, 5th edn. vol. 1 pp. 87–110 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  16. Lachance M.-A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P. 2011; Candida Berkhout (1923). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 pp. 987–1278 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  17. Minter D. M. 2009; Cyberlindnera, a replacement name for Lindnera Kurtzman et al., nom. illegit.. Mycotaxon 110:473–476 [View Article]
    [Google Scholar]
  18. Phaff H. J., Blue J., Hagler A. N., Kurtzman C. P. 1997; Dipodascus starmeri sp. nov., a new species of yeast occurring in cactus necroses. Int J Syst Bacteriol 47:307–312 [View Article][PubMed]
    [Google Scholar]
  19. Romo-Sánchez S., Alves-Baffi M., Arévalo-Villena M., Ubeda-Iranzo J., Briones-Pérez A. 2010; Yeast biodiversity from oleic ecosystems: study of their biotechnological properties. Food Microbiol 27:487–492 [View Article][PubMed]
    [Google Scholar]
  20. Servili M., Selvaggini R., Esposto S., Taticchi A., Montedoro G., Morozzi G. 2004; Health and sensory properties of virgin olive oil hydrophilic phenols: agronomic and technological aspects of production that affect their occurrence in the oil. J Chromatogr A 1054:113–127[PubMed] [CrossRef]
    [Google Scholar]
  21. Servili M., Rizzello C. G., Taticchi A., Esposto S., Urbani S., Mazzacane F., Di Maio I., Selvaggini R., Gobbetti M., Di Cagno R. 2011; Functional milk beverage fortified with phenolic compounds extracted from olive vegetation water, and fermented with functional lactic acid bacteria. Int J Food Microbiol 147:45–52 [View Article][PubMed]
    [Google Scholar]
  22. Swofford D. L. 2002; paup*: phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates;
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  24. Vichi S., Romero A., Tous J., Caixach J. 2011; The activity of healthy olive microbiota during virgin olive oil extraction influences oil chemical composition. J Agric Food Chem 59:4705–4714 [View Article][PubMed]
    [Google Scholar]
  25. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications pp. 315–322 Edited by Innis N., Gelfand D., Sninsky J., White T. London: Academic Press;
    [Google Scholar]
  26. Zullo B. A., Cioccia G., Ciafardini G. 2010; Distribution of dimorphic yeast species in commercial extra virgin olive oil. Food Microbiol 27:1035–1042 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038794-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038794-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error