1887

Abstract

A thorough phenotypic and genotypic analysis of 150 strains belonging to the complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species , three sqvs were detected in the 16S rRNA gene, six sqvs in the gene and 15 sqvs in the gene; in two sqvs were present in each of the three genetic regions; in four, two and nine sqvs were found, respectively, and in three, four and six sqvs were found, respectively. The inappropriate inclusion of within the complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, and genes. sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to . The type strain of this species, of which eight strains were investigated, is ATCC 27353 ( = DSM 45694). A cluster of 24 strains was the basis for the description of sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to with respect to the 16S rRNA gene, sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432 ( = LMG 24735 = CECT 7509). sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the complex, clearly divergent from any other species. The type strain is DSM 45394 ( = CCUG 58460).

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2013-02-01
2024-03-28
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References

  1. Adékambi T., Drancourt M. ( 2004 ). Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. . Int J Syst Evol Microbiol 54, 20952105. [View Article] [PubMed]
    [Google Scholar]
  2. Adékambi T., Colson P., Drancourt M. ( 2003 ). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. . J Clin Microbiol 41, 56995708. [View Article] [PubMed]
    [Google Scholar]
  3. CDC (1996). Standardized Method for HPLC Identification of Mycobacteria. Atlanta: U.S. Department of Health and Human Services, Public Health Service.
  4. Cloud J. L., Meyer J. J., Pounder J. I., Jost K. C. Jr, Sweeney A., Carroll K. C., Woods G. L. ( 2006 ). Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens. . Int J Syst Evol Microbiol 56, 14131418. [View Article] [PubMed]
    [Google Scholar]
  5. CLSI ( 2011 ). Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard, , 2nd edn.. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  6. Devulder G., Pérouse de Montclos M., Flandrois J. P. ( 2005 ). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. . Int J Syst Evol Microbiol 55, 293302. [View Article] [PubMed]
    [Google Scholar]
  7. Kazda J., Cooney R., Monaghan M., Quinn P. J., Stackebrandt E., Dorsch M., Daffé M., Müller K., Cook B. R., Tarnok Z. S. ( 1993 ). Mycobacterium hiberniae sp. nov. . Int J Syst Bacteriol 43, 352357. [View Article] [PubMed]
    [Google Scholar]
  8. Kent P. T., Kubica G. P. ( 1985 ). Public Health Mycobacteriology. A Guide for the Level III Laboratory. Atlanta:: U.S. Department of Health and Human Services;.
    [Google Scholar]
  9. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  10. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. ( 1993 ). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. . J Clin Microbiol 31, 28822889.[PubMed]
    [Google Scholar]
  11. Korsak T., Boisvert H. ( 1972 ). Mycobactéries à pigment rose. . Ann Inst Pasteur (Paris) 122, 3141 (in French).[PubMed]
    [Google Scholar]
  12. Kubica G. P., Silcox V. A., Kilburn J. O., Smithwick R. W., Beam R. E., Jones W. D., Stottmeier K. D. ( 1970 ). Differential identification of mycobacteria. VI. Mycobacterium triviale Kubica sp. nov.. Int J Syst Bacteriol 20, 161174. [View Article]
    [Google Scholar]
  13. Lee H., Lee S. A., Lee I. K., Yu H. K., Park Y. G., Jeong J., Lee S. H., Kim S. R., Hyun J. W. & other authors ( 2010 ). Mycobacterium paraterrae sp. nov. recovered from a clinical specimen: novel chromogenic slow growing mycobacteria related to Mycobacterium terrae complex. . Microbiol Immunol 54, 4653. [View Article] [PubMed]
    [Google Scholar]
  14. Masaki T., Ohkusu K., Hata H., Fujiwara N., Iihara H., Yamada-Noda M., Nhung P. H. H. M., Hayashi M., Asano Y. & other authors ( 2006 ). Mycobacterium kumamotonense sp. nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan: novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. . Microbiol Immunol 50, 889897.[PubMed] [CrossRef]
    [Google Scholar]
  15. McNabb A., Eisler D., Adie K., Amos M., Rodrigues M., Stephens G., Black W. A., Isaac-Renton J. ( 2004 ). Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. . J Clin Microbiol 42, 30003011. [View Article] [PubMed]
    [Google Scholar]
  16. Mignard S., Flandrois J. P. ( 2008 ). A seven-gene, multilocus, genus-wide approach to the phylogeny of mycobacteria using supertrees. . Int J Syst Evol Microbiol 58, 14321441. [View Article] [PubMed]
    [Google Scholar]
  17. Mun H. S., Park J. H., Kim H., Yu H. K., Park Y. G., Cha C. Y., Kook Y. H., Kim B. J. ( 2008 ). Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex. . Int J Syst Evol Microbiol 58, 641646. [View Article] [PubMed]
    [Google Scholar]
  18. Reischl U., Emler S., Horak Z., Kaustova J., Kroppenstedt R. M., Lehn N., Naumann L. ( 1998 ). Mycobacterium bohemicum sp. nov., a new slow-growing scotochromogenic mycobacterium. . Int J Syst Bacteriol 48, 13491355. [View Article] [PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  20. Springer B., Stockman L., Teschner K., Roberts G. D., Böttger E. C. ( 1996 ). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. . J Clin Microbiol 34, 296303.[PubMed]
    [Google Scholar]
  21. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. & other authors ( 2002 ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52, 10431047. [View Article] [PubMed]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  23. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. ( 1993 ). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. . J Clin Microbiol 31, 175178.[PubMed]
    [Google Scholar]
  24. Tsukamura M. ( 1965 ). A group of mycobacteria from soil sources resembling nonphotochromogens (group 3). A description of Mycobacterium nonchromogenicum . . Igaku To Seibutsugaku 71, 110113 (in Japanese).[PubMed]
    [Google Scholar]
  25. Tsukamura M., Kita N., Otsuka W., Shimoide H. ( 1983 ). A study of the taxonomy of the Mycobacterium nonchromogenicum complex and report of six cases of lung infection due to Mycobacterium nonchromogenicum . . Microbiol Immunol 27, 219236.[PubMed] [CrossRef]
    [Google Scholar]
  26. Wayne L. G. ( 1966 ). Classification and identification of mycobacteria. 3. Species within group 3. . Am Rev Respir Dis 93, 919928.[PubMed]
    [Google Scholar]
  27. Wayne L. G., Kubica G. P. ( 1986 ). Family Mycobacteriaceae CHESTER 1897, 63AL . . In Bergey’s Manual of Systematic Bacteriology, pp. 14351457. Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. . Baltimore:: The Williams & Wilkins Co;.
    [Google Scholar]
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