1887

Abstract

Phylogenetic analysis of the 16S rRNA gene sequences of strains TE26 and K6 belonging to Kageyama 2005 showed the strains to be deeply intermingled among the species of the genus . The comparison showed that strain TE26 was closely related to the type strains of (99.1 % 16S rRNA gene sequence similarity), (98.7 %), (98.7 %) and (98.0 %). However, DNA–DNA hybridization experiments (less than 20 % relatedness) demonstrated that strain TE26 is different from these species. A comparative phenotypic and chemotaxonomic analysis (based on fatty acid profiles) in combination with the 16S rRNA gene sequence phylogenetic analysis and the DNA–DNA hybridization results supported the incorporation of into the genus as comb. nov.; the type strain is TE26 ( = LMG 26411  = DSM 15562  = CIP 108892).

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2013-01-01
2019-10-19
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References

  1. Anisimova M. , Gascuel O. . ( 2006; ). Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. . Syst Biol 55:, 539–552. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chen W. M. , Moulin L. , Bontemps C. , Vandamme P. , Béna G. , Boivin-Masson C. . ( 2003; ). Legume symbiotic nitrogen fixation by β-proteobacteria is widespread in nature. . J Bacteriol 185:, 7266–7272. [CrossRef] [PubMed]
    [Google Scholar]
  4. Coenye T. , Falsen E. , Vancanneyt M. , Hoste B. , Govan J. R. W. , Kersters K. , Vandamme P. . ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov.. Int J Syst Bacteriol 49:, 405–413. [CrossRef] [PubMed]
    [Google Scholar]
  5. Coenye T. , Vandamme P. , Govan J. R. , LiPuma J. J. . ( 2001; ). Taxonomy and identification of the Burkholderia cepacia complex. . J Clin Microbiol 39:, 3427–3436. [CrossRef] [PubMed]
    [Google Scholar]
  6. Coenye T. , Vandamme P. , LiPuma J. J. . ( 2003; ). Ralstonia respiraculi sp. nov., isolated from the respiratory tract of cystic fibrosis patients. . Int J Syst Evol Microbiol 53:, 1339–1342. [CrossRef] [PubMed]
    [Google Scholar]
  7. Cuadrado V. , Gomila M. , Merini L. , Giulietti A. M. , Moore E. R. B. . ( 2010; ). Cupriavidus pampae sp. nov., a novel herbicide-degrading bacterium isolated from agricultural soil. . Int J Syst Evol Microbiol 60:, 2606–2612. [CrossRef] [PubMed]
    [Google Scholar]
  8. da Silva K. , Florentino L. A. , da Silva K. B. , de Brandt E. , Vandamme P. , de Souza Moreira F. M. . ( 2012; ). Cupriavidus necator isolates are able to fix nitrogen in symbiosis with different legume species. . Syst Appl Microbiol 35:, 175–182. [CrossRef] [PubMed]
    [Google Scholar]
  9. Edgar R. C. . ( 2004; ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32:, 1792–1797. [CrossRef] [PubMed]
    [Google Scholar]
  10. Estrada-de los Santos P. , Bustillos-Cristales R. , Caballero-Mellado J. . ( 2001; ). Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. . Appl Environ Microbiol 67:, 2790–2798. [CrossRef] [PubMed]
    [Google Scholar]
  11. Estrada-de los Santos P. , Vacaseydel-Aceves N. B. , Martínez-Aguilar L. , Cruz-Hernández M. A. , Mendoza-Herrera A. , Caballero-Mellado J. . ( 2011; ). Cupriavidus and Burkholderia species associated with agricultural plants that grow in alkaline soils. . J Microbiol 49:, 867–876. [CrossRef] [PubMed]
    [Google Scholar]
  12. Goris J. , De Vos P. , Coenye T. , Hoste B. , Janssens D. , Brim H. , Diels L. , Mergeay M. , Kersters K. , Vandamme P. . ( 2001; ). Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend.. Int J Syst Evol Microbiol 51:, 1773–1782. [CrossRef] [PubMed]
    [Google Scholar]
  13. Guindon S. , Delsuc F. , Dufayard J. F. , Gascuel O. . ( 2009; ). Estimating maximum likelihood phylogenies with PhyML. . Methods Mol Biol 537:, 113–137. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kageyama C. , Ohta T. , Hiraoka K. , Suzuki M. , Okamoto T. , Ohishi K. . ( 2005a; ). Chlorinated aliphatic hydrocarbon-induced degradation of trichloroethylene in Wautersia numadzuensis sp. nov.. Arch Microbiol 183:, 56–65. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kageyama C. , Ohta T. , Hiraoka K. , Suzuki M. , Okamoto T. , Ohishi K. . ( 2005b; ). Wautersia numazuensis corrig., sp. nov. In Validation of Publication of New Names and New Combinations Previously Effectively Published Outside the IJSEM, Validation List no. 104. . Int J Syst Evol Microbiol 55:, 1395–1397. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  17. Mahenthiralingam E. , Bischof J. , Byrne S. K. , Radomski C. , Davies J. E. , Av-Gay Y. , Vandamme P. . ( 2000; ). DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. . J Clin Microbiol 38:, 3165–3173.[PubMed]
    [Google Scholar]
  18. Makkar N. S. , Casida L. E. Jr . ( 1987; ). Cupriavidus necator gen. nov., sp. nov.: a nonobligate bacterial predator of bacteria in soil. . Int J Syst Bacteriol 37:, 323–326. [CrossRef]
    [Google Scholar]
  19. Sato Y. , Nishihara H. , Yoshida M. , Watanabe M. , Rondal J. D. , Concepcion R. N. , Ohta H. . ( 2006; ). Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines. . Int J Syst Evol Microbiol 56:, 973–978. [CrossRef] [PubMed]
    [Google Scholar]
  20. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. , Kämpfer P. , Maiden M. C. , Nesme X. , Rosselló-Mora R. , Swings J. . & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tayeb L. A. , Lefevre M. , Passet V. , Diancourt L. , Brisse S. , Grimont P. A. D. . ( 2008; ). Comparative phylogenies of Burkholderia, Ralstonia, Comamonas, Brevundimonas and related organisms derived from rpoB, gyrB and rrs gene sequences. . Res Microbiol 159:, 169–177. [CrossRef] [PubMed]
    [Google Scholar]
  23. Vandamme P. , Coenye T. . ( 2004; ). Taxonomy of the genus Cupriavidus: a tale of lost and found. . Int J Syst Evol Microbiol 54:, 2285–2289. [CrossRef] [PubMed]
    [Google Scholar]
  24. Vandamme P. , Dawyndt P. . ( 2011; ). Classification and identification of the Burkholderia cepacia complex: past, present and future. . Syst Appl Microbiol 34:, 87–95. [CrossRef] [PubMed]
    [Google Scholar]
  25. Vandamme P. , Pot B. , Gillis M. , de Vos P. , Kersters K. , Swings J. . ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. . Microbiol Rev 60:, 407–438.[PubMed]
    [Google Scholar]
  26. Vandamme P. , Holmes B. , Vancanneyt M. , Coenye T. , Hoste B. , Coopman R. , Revets H. , Lauwers S. , Gillis M. . & other authors ( 1997; ). Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov.. Int J Syst Bacteriol 47:, 1188–1200. [CrossRef] [PubMed]
    [Google Scholar]
  27. Vandamme P. , Goris J. , Coenye T. , Hoste B. , Janssens D. , Kersters K. , De Vos P. , Falsen E. . ( 1999; ). Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov.. Int J Syst Bacteriol 49:, 663–669.[CrossRef]
    [Google Scholar]
  28. Vaneechoutte M. , Kämpfer P. , De Baere T. , Falsen E. , Verschraegen G. . ( 2004; ). Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.. Int J Syst Evol Microbiol 54:, 317–327. [CrossRef] [PubMed]
    [Google Scholar]
  29. Yang Z. . ( 1996; ). Among-site rate variation and its impact on phylogenetic analyses. . Trends Ecol Evol 11:, 367–372. [CrossRef] [PubMed]
    [Google Scholar]
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