RT Journal Article SR Electronic(1) A1 Carro, Lorena A1 Pukall, Rüdiger A1 Spröer, Cathrin A1 Kroppenstedt, Reiner M. A1 Trujillo, Martha E.YR 2012 T1 Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_12 SP 2971 OP 2977 DO https://doi.org/10.1099/ijs.0.038695-0 PB Microbiology Society, SN 1466-5034, AB Three actinobacterial strains, CR30T, CR36 and CR38T, were isolated from rhizosphere soil of Pisum sativum plants collected in Spain. The strains were filamentous, Gram-stain-positive and produced single spores. Phylogenetic, chemotaxonomic and morphological analyses confirmed that the three strains belonged to the genus Micromonospora . 16S rRNA gene sequence analysis of strains CR30T and CR36 showed a close relationship to Micromonospora coriariae NAR01T (99.3 % similarity) while strain CR38T had a similarity of 99.0 % with Micromonospora saelicesensis Lupac 09T. In addition, gyrB gene phylogeny clearly differentiated the novel isolates from recognized Micromonospora species. DNA–DNA hybridization, BOX-PCR and ARDRA profiles confirmed that these strains represent novel genomic species. The cell-wall peptidoglycan of strains CR30T and CR38T contained meso-diaminopimelic acid. Both strains had MK-10(H4) as the main menaquinone and a phospholipid type II pattern. An array of physiological tests also differentiated the isolates from their closest neighbours. Considering all the data obtained, it is proposed that strains CR30T and CR36 represent a novel species under the name Micromonospora cremea sp. nov. (type strain CR30T = CECT 7891T = DSM 45599T), whereas CR38T represents a second novel species, for which the name Micromonospora zamorensis sp. nov. is proposed, with CR38T ( = CECT 7892T = DSM 45600T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.038695-0