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Volume 62, Issue Pt_9

sp. nov., a psychrotolerant bacterium isolated from Arctic marine sediment Free

Abstract

A novel Gram-stain-negative, horseshoe-shaped, non-motile bacterium, designated strain M12-11B, was isolated from a marine sediment sample collected at a depth of 200 m from Kongsfjorden, Svalbard. The colony colour was orangish red due to the presence of carotenoids. Fatty acids were dominated by branched and unsaturated fatty acids (90.8 %), with a high abundance of iso-C (14.9 %), anteiso-C (11.4 %), iso-C G (13.1 %), Cω6 (5.4 %), Cω6 (6.7 %), summed feature 3 (Cω7 and/or Cω6; 9.3 %) and summed feature 9 (10-methyl C and/or iso-Cω9; 5.9 %). Strain M12-11B contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, one unidentified aminolipid and three unidentified lipids. Based on 16S rRNA gene sequence similarities, the type strains of , and were most closely related to M12-11B with sequence similarities of 98.2, 96.8 and 93.3 %, respectively. Other members of the family had sequence similarities of <92.0 %. However, DNA–DNA hybridization with KCTC 12363 and DSM 745 showed relatedness values of only 24.5 and 32.5 % with respect to strain M12-11B. Based on the results of DNA–DNA hybridization experiments and phenotypic and chemotaxonomic data, it appears that strain M12-11B represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is M12-11B ( = KCTC 23011 = NBRC 106168) and it has a DNA G+C content of 40.5 mol%.

  • Published Online:
Funding
This study was supported by the:
  • National Centre for Antarctic and Ocean Research, Goa
  • CSIR Network Project on Exploitation of India’s rich microbial diversity (Award NWP0006)
  • CSIR, Government of India
© 2012 IUMS
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2012-09-01
2024-04-19
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Cyclobacterium qasimii sp. nov., a psychrotolerant bacterium isolated from Arctic marine sediment
Int J Syst Evol Microbiol 62, 2133 (2012); https://doi.org/10.1099/ijs.0.038661-0
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