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Volume 63, Issue Pt_1

sp. nov., co-isolated with a colonial choanoflagellate Open Access

Abstract

A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1, was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1 grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1 contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C (29.4 %), iso-Cω9 (18.5 %) and summed feature 3 (Cω6 and/or Cω7; 11.3 %). The major polar lipids detected in strain PR1 were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1 fell within the cluster comprising the genus and was most closely related to JC 2051 (95.4 % sequence similarity) and S1-3 (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1 and the type strains of other species of the genus were in the range 91–95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1 demonstrated that this strain was distinct from other members of the genus , including its closest relative, . Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1 should be placed in the genus as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is PR1 ( = ATCC BAA-2233  = DSM 24695).

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Funding
This study was supported by the:
  • Gordon and Betty Moore Foundation
  • NIH (Award 5F32GM086054)
  • NASA Astrobiology Institute (Award NNA08CN84A))
© 2013 IUMS
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2013-01-01
2024-04-19
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Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate
Int J Syst Evol Microbiol 63, 163 (2013); https://doi.org/10.1099/ijs.0.038646-0
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