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Volume 62, Issue Pt_9

gen. nov., sp. nov., a haloalkalitolerant bacterium of the family , isolated from a haloalkaline lake and emended descriptions of the genera , and Free

Abstract

A novel Gram-staining-negative, rod-shaped, non-motile bacterium, designated strain LW9, was isolated from a water sample collected from Lonar Lake of Buldhana district, Maharashtra, India. Colonies and broth cultures were reddish orange due to the presence of carotenoid pigments. Strain LW9 was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. The predominant fatty acids were iso-C (31.3 %), iso-C (9.3 %), anteiso-C (7.3 %), iso-C H (6.1 %), summed feature 3 (comprising Cω7/Cω6; 5.9 %), iso-Cω9 (5.4 %) and iso-C 3-OH (5.0 %). Strain LW9 contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and seven unidentified lipids. The DNA G+C content of strain LW9 was 40.5 mol%. 16S rRNA gene sequence analysis indicated that the type strains of and , two members of the family (phylum ‘’) were the most closely related strains with sequence similarities of 93.0 and 94.0 %, respectively. Other members of the family showed sequence similarities <93.0 %. Based on these phenotypic characteristics and on phylogenetic inference, strain LW9 is proposed as the representative of novel species in a new genus, gen. nov., sp. nov. The type strain of the type species, , is LW9 ( = CCUG 58316 = KCTC 22772). Emended descriptions of the genera , and are also proposed.

  • Published Online:
Funding
This study was supported by the:
  • National Centre for Antarctic and Ocean Research, Goa, Department of Biotechnology
  • CSIR Network Project on Exploitation of India’s rich microbial diversity (Award NWP0006)
  • CSIR, Government of India
© 2012 IUMS
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2012-09-01
2024-04-25
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Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera Indibacter, Nitritalea and Belliella
Int J Syst Evol Microbiol 62, 2252 (2012); https://doi.org/10.1099/ijs.0.038604-0
/content/journal/ijsem/10.1099/ijs.0.038604-0
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