1887

Abstract

A strain, PEPV15, was isolated from a nodule on grown in soil in northern Spain. Phylogenetic analyses of 16S rRNA and genes showed that this strain belongs to the genus The most closely related species were, in both cases, , and , the type strains of which gave sequence similarities of 98.9, 98.6 and 98.4 %, respectively, in the 16S rRNA gene and 88.1, 87.5 and 88.7 %, respectively, in the gene. PEPV15 contained Q-10 as the major quinone (88 %) and low amounts of Q-9 (12 %). It differed from its closest relatives in its growth in diverse culture conditions and in the assimilation of several carbon sources. The strain was not able to produce nodules in The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus for which the name sp. nov. is proposed; the type strain is PEPV15 ( = LMG 26470 = CECT 7949). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Ministerio de Ciencia e Innovación (MICINN)
  • Junta de Castilla y León
  • Universidad de Salamanca
  • Junta para la Ampliación de Estudios (JAE)-Doc (Consejo Superior de Investigaciones Científicas
  • MICINN
  • Caroline Foundation
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2013-03-01
2024-04-19
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