A Gram-reaction-negative, rod-shaped, gliding and bright-yellow-pigmented bacterial strain, designated JCN-23T, was isolated from a soil sample collected from an arid area in Gansu Province in north-west China, and characterized by using a polyphasic taxonomic approach. This isolate grew optimally at 30 °C and in the absence of NaCl. The only respiratory quinone was menaquinone-7 and the major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The only polyamine was homospermidine and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 47.1 mol%. Comparative 16S rRNA gene sequence analysis showed that strain JCN-23T was a member of the phylum Bacteroidetes, exhibiting the highest 16S rRNA gene sequence similarity to Flavitalea populi CCTCC AB 208255T (97.6 %). No other recognized bacterial species showed more than 93.4 % 16S rRNA gene sequence similarity to the novel isolate. DNA–DNA hybridization experiments showed a low level (26 %) of DNA–DNA relatedness between strain JCN-23T and F. populi CCTCC AB 208255T. On the basis of the phenotypic and genotypic data and phylogenetic inference, strain JCN-23T is considered to represent a novel species of the genus Flavitalea, for which the name Flavitalea gansuensis sp. nov. is proposed. The type strain is JCN-23T ( = ACCC 05418T = KCTC 23071T). Emended descriptions of the genus Flavitalea and Flavitalea populi are also proposed.
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Funding
This study was supported by the:
National Natural Science Foundation of Chin
(Award 31100001)
China Postdoctoral Science Foundation
(Award 20100481373)
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