1887

Abstract

A Gram-negative, motile, facultatively anaerobic rod, designated A36, was isolated from a dead ark clam found on the south coast of Korea. The isolate was catalase- and oxidase-negative. 16S rRNA gene sequence analysis indicated that strain A36 was most closely related to KMD 001, with which it shared 98.2 % 16S rRNA gene sequence similarity. Strain A36 grew optimally at 30–37 °C, with 1 % (w/v) NaCl and at pH 8.0. The major respiratory quinone was ubiquinone-9 (Q-9). The major polar lipids were phosphatidylserine, phosphoethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were summed feature 3 (comprising Cω7 and/or iso-C 2-OH) and C. The genomic DNA G+C content was 47.3 mol%. DNA–DNA relatedness between the isolate and JCM 15607 was <25±3 %. Strain A36 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A36 ( = KACC 16204  = JCM 17805). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , National Fisheries Research and Development Institute (NFRDI)
  • , National Institute of Biological Resources (NIBR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038422-0
2012-12-01
2020-07-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/2865.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038422-0&mimeType=html&fmt=ahah

References

  1. Bae J.-W., Rhee S.-K., Park J. R., Chung W.-H., Nam Y.-D., Lee I., Kim H., Park Y.-H. 2005; Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. Appl Environ Microbiol 71:8825–8835 [CrossRef][PubMed]
    [Google Scholar]
  2. Chang H.-W., Nam Y.-D., Jung M. Y., Kim K.-H., Roh S. W., Kim M.-S., Jeon C. O., Yoon J.-H., Bae J.-W. 2008; Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 75:523–530 [CrossRef][PubMed]
    [Google Scholar]
  3. Choi E. J., Kwon H. C., Sohn Y. C., Yang H. O. 2010; Kistimonas asteriae gen. nov., sp. nov., a gammaproteobacterium isolated from Asteria samurensis . Int J Syst Evol Microbiol 60:938–943 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D. 1981a; A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Jones D. 1981b; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354[PubMed]
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [CrossRef][PubMed]
    [Google Scholar]
  8. Kämpfer P., Rosselló-Mora R., Hermansson M., Persson F., Huber B., Falsen E., Busse H. J. 2007; Undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 57:1510–1515 [CrossRef][PubMed]
    [Google Scholar]
  9. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  10. MIDI 1999 Sherlock Microbial Identification System Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  11. Rochelle P. A., Fry J. C., Parkes R. J., Weightman A. J. 1992; DNA extraction for 16S rRNA gene analysis to determine genetic diversity in deep sediment communities. FEMS Microbiol Lett 79:59–65[PubMed] [CrossRef]
    [Google Scholar]
  12. Roh S. W., Sung Y., Nam Y.-D., Chang H.-W., Kim K.-H., Yoon J.-H., Jeon C. O., Oh H.-M., Bae J.-W. 2008; Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46:40–44 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  17. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  18. Tittsler R. P., Sandholzer L. A. 1936; The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580[PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  20. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. 2000; Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038422-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038422-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error