1887

Abstract

A strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9, was isolated from rhizosphere soil of the medicinal herb . Strain DR-9 grew at 20–40 °C, at pH 4.0–9.0 and in the presence of 0–1 % (w/v) NaCl. The major fatty acids were iso-C and summed feature 3 (Cω7 and/or Cω6 and/or iso-C 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9 formed a lineage within the genus and was closely related to DRP28 (96.1 % sequence similarity), HMD1056 (95.9 % sequence similarity), XM-003 (95.8 %) and BDR-9 (95.1 %). The status of strain DR-9 as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9 % DNA–DNA relatedness with DRP28, XM-003 and BDR-9, respectively. The genomic DNA G+C content of strain DR-9 was 49.8 %. These data suggest that strain DR-9 should be considered as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DR-9 ( = KACC 16469 = NBRC 108839).

Funding
This study was supported by the:
  • , National Academy of Agricultural Science
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2013-08-01
2021-01-19
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