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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_11

gen. nov., sp. nov., a member of the family isolated from estuarine water Free

Abstract

A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1, was isolated from an estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2.0–2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Strain K1 was negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction and HS production. The fatty acids were dominated by iso-branched components with a high abundance of iso-C, iso-C G and iso-C 3-OH; MK-6 (64 %) and MK-7 (34 %) were found as major respiratory quinones; and phosphatidylethanolamine, two unidentified aminolipids, four unidentified phospholipids and two unidentified lipids were major polar lipids. The DNA G+C content of strain K1 was 46.1 mol%. 16S rRNA gene sequence analysis indicated that strain K1 was related most closely to the type strain of (pairwise sequence similarity of 93.0 %). Phylogenetic analysis showed that strain K1 formed a distinct branch within the family and clustered with the clade comprising species of the genera , and , being phylogenetically most closely related to the type strain of at a distance of 9.2 % (90.8 % similarity). Other species of the genera within the same clade were related to strain K1 at distances of 15.0–23.1 %. Based on phenotypic and chemotaxonomic characteristics and on phylogenetic inference, strain K1 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is K1 ( = MTCC 11055 = JCM 17677).

  • Published Online:
Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR)
  • Department of Biotechnology, Government of India
© 2012 IUMS
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2012-11-01
2025-03-23
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Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water
Int J Syst Evol Microbiol 62, 2624 (2012); https://doi.org/10.1099/ijs.0.038356-0
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