1887

Abstract

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0264, was isolated from soil sampled in Mongolia. The isolate formed pale to moderate yellowish brown colonies and branched substrate mycelium. On the basis of 16S rRNA gene sequence analysis, strain MN08-A0264 belonged to the genus and exhibited 97.9 % 16S rRNA gene sequence similarity with IMSNU 22120, 97.7 % with IFO 15962, 97.2 % with IFO 15965 and 96.8 % with IFO 15966. The allocation of the isolate to the genus was supported by chemotaxonomic data: menaquinone MK-9(H) with minor amounts of MK-9(H) and MK-9(H), major amounts of iso-C, C9 and C 10-methyl, a polar lipid profile comprising phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and glycolipids, and whole-cell sugars glucose, galactose, acofriose ( methylrhamnose), mannose, ribose, arabinose, xylose and rhamnose (trace). DNA–DNA relatedness (5–20 %) differentiated the isolate from its closest neighbours. The physiological and biochemical tests allowed the differentiation of strain MN08-A0264 from members of the genus . Thus, strain MN08-A0264 represents a novel species, for which the name sp. nov. is proposed. The type strain is MN08-A0264 ( = NBRC 105887  = VTCC D9-27).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038307-0
2012-10-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/10/2480.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038307-0&mimeType=html&fmt=ahah

References

  1. Adachi J. , Hasegawa M. . ( 1995; ). Improved dating of the human/chimpanzee separation in the mitochondrial DNA tree: heterogeneity among amino acid sites. . J Mol Evol 40:, 622–628. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  3. Eck R. V. , Dayhoff M. O. . ( 1966; ). Atlas of Protein Sequences and Structure. Silver Springs, MD:: National Biomedical Research Foundation;.
    [Google Scholar]
  4. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Gordon R. E. , Mihm J. M. . ( 1957; ). A comparative study of some strains received as nocardiae. . J Bacteriol 73:, 15–27.[PubMed]
    [Google Scholar]
  7. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  8. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  9. Hasegawa M. , Kishino H. , Yano T. . ( 1985; ). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. . J Mol Evol 22:, 160–174. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hayakawa M. , Nonomura H. . ( 1987; ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  11. Hayakawa M. , Otoguro M. , Takeuchi T. , Yamazaki T. , Iimura Y. . ( 2000; ). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. . Antonie van Leeuwenhoek 78:, 171–185. [CrossRef] [PubMed]
    [Google Scholar]
  12. Humble M. W. , King A. , Phillips I. . ( 1977; ). API ZYM: a simple rapid system for the detection of bacterial enzymes. . J Clin Pathol 30:, 275–277. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kloos W. E. , Tornabene T. G. , Schleifer K. H. . ( 1974; ). Isolation and characterization of Micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae . . Int J Syst Bacteriol 24:, 79–101. [CrossRef]
    [Google Scholar]
  14. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Mori K. , Kim H. , Kakegawa T. , Hanada S. . ( 2003; ). A novel lineage of sulfate-reducing microorganisms: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense, gen. nov., sp. nov., a new thermophilic isolate from a hot spring. . Extremophiles 7:, 283–290. [CrossRef] [PubMed]
    [Google Scholar]
  18. Nozawa Y. , Sakai N. , Arai K. , Kawasaki Y. , Harada K. . ( 2007; ). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey’s method. . J Microbiol Methods 70:, 306–311. [CrossRef] [PubMed]
    [Google Scholar]
  19. Ruan J.-S. , Lechevalier M. P. , Jiang C.-R. , Lechevalier H. A. . ( 1985; ). A new species of the genus Actinoplanes, Actinoplanes minutisporangius n. sp.. Actinomycetes 19:, 163–175.
    [Google Scholar]
  20. Rzhetsky A. , Nei M. . ( 1992; ). A simple method for estimating and testing minimum evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  21. Saito H. , Miura K. I. . ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef] [PubMed]
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  25. Stackebrandt E. , Rainey F. A. , Ward-Rainey N. L. . ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:, 479–491. [CrossRef]
    [Google Scholar]
  26. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  27. Tamura T. , Hatano K. . ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:, 2119–2125. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tamura T. , Nakagaito Y. , Nishii T. , Hasegawa T. , Stackebrandt E. , Yokota A. . ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:, 193–203. [CrossRef] [PubMed]
    [Google Scholar]
  29. Tamura T. , Hayakawa M. , Hatano K. . ( 1998; ). A new genus of the order Actinomycetales, Cryptosporangium gen. nov., with descriptions of Cryptosporangium arvum sp. nov. and Cryptosporangium japonicum sp. nov.. Int J Syst Bacteriol 48:, 995–1005. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evoloutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  31. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  32. Zhi X.-Y. , Li W.-J. , Stackebrandt E. . ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59:, 589–608. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038307-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038307-0
Loading

Data & Media loading...

Supplements

Supplementary data 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error