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Abstract

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0264, was isolated from soil sampled in Mongolia. The isolate formed pale to moderate yellowish brown colonies and branched substrate mycelium. On the basis of 16S rRNA gene sequence analysis, strain MN08-A0264 belonged to the genus and exhibited 97.9 % 16S rRNA gene sequence similarity with IMSNU 22120, 97.7 % with IFO 15962, 97.2 % with IFO 15965 and 96.8 % with IFO 15966. The allocation of the isolate to the genus was supported by chemotaxonomic data: menaquinone MK-9(H) with minor amounts of MK-9(H) and MK-9(H), major amounts of iso-C, C9 and C 10-methyl, a polar lipid profile comprising phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and glycolipids, and whole-cell sugars glucose, galactose, acofriose ( methylrhamnose), mannose, ribose, arabinose, xylose and rhamnose (trace). DNA–DNA relatedness (5–20 %) differentiated the isolate from its closest neighbours. The physiological and biochemical tests allowed the differentiation of strain MN08-A0264 from members of the genus . Thus, strain MN08-A0264 represents a novel species, for which the name sp. nov. is proposed. The type strain is MN08-A0264 ( = NBRC 105887  = VTCC D9-27).

Funding
This study was supported by the:
  • Deanship of Scientific Research at King Saud University (Award RGP-VPP-072)
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2012-10-01
2024-03-29
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