1887

Abstract

A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18, was isolated from a water sample collected from the River Mahananda at Siliguri (26° 44′ 23.20′ N, 88° 25′ 22.89′ E), West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was NCDO 2286 (96 % similarity). The DNA G+C content of strain MB18 was 64.6 mol%. Chemotaxonomic data [MK-8(H) as the major menaquinone, galactose as the sole cell-wall sugar, -diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C, anteiso-C and iso-C as the major fatty acids] supported the affiliation of strain MB18 to the genus . The results of DNA G+C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18 from its nearest neighbour . The isolate therefore represents a novel species, for which the name sp. nov. is proposed; the type strain is MB18 ( = DSM 23676 = LMG 25772).

Funding
This study was supported by the:
  • Department of Biotechnology (DBT), Government of India (Award BT/PR-7164/BCE/08/448/2006 and DBT-JRF/05-06/71/29.08.05)
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2013-02-01
2022-01-20
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