1887

Abstract

A Gram-positive-staining, rod-shaped, facultatively oligotrophic bacterial strain, designated MB18, was isolated from a water sample collected from the River Mahananda at Siliguri (26° 44′ 23.20′ N, 88° 25′ 22.89′ E), West-Bengal, India. On the basis of 16S rRNA gene sequence similarity, the closest relative of this strain was NCDO 2286 (96 % similarity). The DNA G+C content of strain MB18 was 64.6 mol%. Chemotaxonomic data [MK-8(H) as the major menaquinone, galactose as the sole cell-wall sugar, -diaminopimelic acid as the diagnostic cell-wall diamino acid, phosphatidylglycerol and diphosphatidylglycerol as constituents of the polar lipids, anteiso-C, anteiso-C and iso-C as the major fatty acids] supported the affiliation of strain MB18 to the genus . The results of DNA G+C content, 16S rRNA gene sequence analysis and biochemical and physiological analyses allowed genotypic and phenotypic differentiation of strain MB18 from its nearest neighbour . The isolate therefore represents a novel species, for which the name sp. nov. is proposed; the type strain is MB18 ( = DSM 23676 = LMG 25772).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038281-0
2013-02-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/511.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038281-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  3. Boone C. J. , Pine L. . ( 1968; ). Rapid method for characterization of actinomycetes by cell wall composition. . Appl Microbiol 16:, 279–284.[PubMed]
    [Google Scholar]
  4. Bouvet P. J. M. , Grimont P. A. D. . ( 1986; ). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii . . Int J Syst Bacteriol 36:, 228–240. [CrossRef]
    [Google Scholar]
  5. Breed R. S. . ( 1957; ). Family IX. Brevibacteriaceae Breed, 1953. . In Bergey's Manual of Determinative Bacteriology, , 7th edn., pp. 490–505. Edited by Breed R. S. , Murray E. G. D. , Smith N. R. . . London:: Baillière, Tindall & Cox;.
    [Google Scholar]
  6. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  7. Claus D. . ( 1992; ). A standardized Gram staining procedure. . World J Microb Biot 8:, 451–452. [CrossRef]
    [Google Scholar]
  8. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  9. Collins M. D. , Jones D. , Keddie R. M. , Sneath P. H. A. . ( 1980; ). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev., comb. nov.. J Gen Microbiol 120:, 1–10.
    [Google Scholar]
  10. Collins M. D. , Farrow J. A. E. , Goodfellow M. , Minnikin D. E. . ( 1983; ). Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov.. Syst Appl Microbiol 4:, 388–395. [CrossRef]
    [Google Scholar]
  11. Counsell T. J. , Murray R. G. E. . ( 1986; ). Polar lipid profiles of the genus Deinococcus . . Int J Syst Bacteriol 36:, 202–206. [CrossRef]
    [Google Scholar]
  12. Cui Y. , Kang M.-S. , Woo S.-G. , Jin L. , Kim K. K. , Park J. , Lee M. , Lee S.-T. . ( 2012; ). Brevibacterium daeguense sp. nov. a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture. . Int J Syst Evol Microbiol. [CrossRef] [PubMed]
    [Google Scholar]
  13. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  14. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  15. Gordon R. E. , Barnett D. A. , Handerhan J. E. , Pang C. H.-N. . ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  16. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kumar A. , Mukherjee S. , Chakraborty R. . ( 2010; ). Characterization of a novel trimethoprim resistance gene, dfrA28, in class 1 integron of an oligotrophic Acinetobacter johnsonii strain, MB52, isolated from River Mahananda, India. . Microb Drug Resist 16:, 29–37. [CrossRef] [PubMed]
    [Google Scholar]
  19. Lee S. D. . ( 2006; ). Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. . Int J Syst Evol Microbiol 56:, 1889–1892. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic-acid by high-performance liquid-chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E. , Hutchinson I. G. , Caldicott A. B. , Goodfellow M. . ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188:, 221–233. [CrossRef]
    [Google Scholar]
  22. Minnikin D. E. , Odonnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  23. Murray P. R. , Baron E. J. , Pfaller M. A. , Tenover F. C. , Yolken R. H. . (editors) ( 1999; ). Manual of Clinical Microbiology, , 7th edn.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  24. Pearson W. R. . ( 1990; ). Rapid and sensitive sequence comparison with FASTP and FASTA.. Methods Enzymol 183:, 63–98. [CrossRef] [PubMed]
    [Google Scholar]
  25. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  27. Staneck J. L. , Roberts G. D. . ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  28. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  30. Wauters G. , Van Bosterhaut B. , Janssens M. , Verhaegen J. . ( 1998; ). Identification of Corynebacterium amycolatum and other nonlipophilic fermentative corynebacteria of human origin. . J Clin Microbiol 36:, 1430–1432.[PubMed]
    [Google Scholar]
  31. Wauters G. , Charlier J. , Janssens M. , Delmée M. . ( 2001; ). Brevibacterium paucivorans sp. nov., from human clinical specimens. . Int J Syst Evol Microbiol 51:, 1703–1707. [CrossRef] [PubMed]
    [Google Scholar]
  32. Wauters G. , Avesani V. , Laffineur K. , Charlier J. , Janssens M. , Van Bosterhaut B. , Delmée M. . ( 2003; ). Brevibacterium lutescens sp. nov., from human and environmental samples. . Int J Syst Evol Microbiol 53:, 1321–1325. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038281-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038281-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error