1887

Abstract

The pigeon pea witches’-broom phytoplasma group (16SrIX) comprises diverse strains that cause numerous diseases in leguminous trees and herbaceous crops, vegetables, a fruit, a nut tree and a forest tree. At least 14 strains have been reported worldwide. Comparative phylogenetic analyses of the highly conserved 16S rRNA gene and the moderately conserved ()– () and genes indicated that the 16SrIX group consists of at least six distinct genetic lineages. Some of these lineages cannot be readily differentiated based on analysis of 16S rRNA gene sequences alone. The relative genetic distances among these closely related lineages were better assessed by including more variable genes [e.g. ribosomal protein (rp) and genes]. The present study demonstrated that virtual RFLP analyses using rp and gene sequences allowed unambiguous identification of such lineages. A coding system is proposed to designate each distinct rp and subgroup in the 16SrIX group.

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2012-09-01
2019-12-13
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