A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were Brevibacterium pityocampae DSM 21720T (97.2 %), Brevibacterium salitolerans KCTC 19616T (96.7 %), Brevibacterium album KCTC 19173T (96.2 %) and Brevibacterium samyangense KCCM 42316T (96.2 %). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15 : 0, anteiso-C17 : 0, C16 : 0 and iso-C15 : 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus Brevibacterium. The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA–DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus Brevibacterium. The isolate is therefore considered to represent a novel species in the genus Brevibacterium, for which the name Brevibacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T ( = KCTC 19800T = JCM 17458T).
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Funding
This study was supported by the:
GAIA Project, Ministry of Environment, Republic of Korea
(Award 173-091-006)
BaeH. S.,
LeeJ. M.,
KimY. B.,
LeeS.-T.(1996). Biodegradation of the mixtures of 4-chlorophenol and phenol by Comamonas testosteroni CPW301. . Biodegradation7, 463–469. [View Article][PubMed]
BreedR. S.(1953). The families developed from Bacteriaceae Cohn with a description of the family Brevibacteriaceae. Riass Commun VI Congr Int Microbiol Roma 1, 10–15.
BuckJ. D.(1982). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol44, 992–993.[PubMed]
ColeJ. R.,
CascarelliA. L.,
MohnW. W.,
TiedjeJ. M.(1994). Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol. . Appl Environ Microbiol60, 3536–3542.[PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
HallT. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser41, 95–98.
KämpferP.,
SchäferJ.,
LoddersN.,
BusseH.-J.(2010).Brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment. . Int J Syst Evol Microbiol60, 909–913. [View Article][PubMed]
MadsenT.,
AamandH.(1992). Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture. . Appl Environ Microbiol58, 557–561.[PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MikesellM. D.,
BoydS. A.(1986). Complete reductive dechlorination and mineralization of pentachlorophenol by anaerobic microorganisms. . Appl Environ Microbiol52, 861–865.[PubMed]
MinnikinD. E.,
PatelP. V.,
AlshamaonyL.,
GoodfellowM.(1977). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol27, 104–117. [View Article]
MooreD. D.,
DowhanD.(1995). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
. New York:: Wiley;.
SasserM.(1990).Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
SeiK.,
AsanoK.-I.,
TateishiN.,
MoriK.,
IkeM.,
FujitaM.(1999). Design of PCR primers and gene probes for the general detection of bacterial populations capable of degrading aromatic compounds via catechol cleavage pathways. . J Biosci Bioeng88, 542–550. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]