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Volume 63, Issue Pt_1

sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture Free

Abstract

A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41 were DSM 21720 (97.2 %), KCTC 19616 (96.7 %), KCTC 19173 (96.2 %) and KCCM 42316 (96.2 %). The DNA G+C content of strain 2C6-41 was 66.4 mol%. Chemotaxonomic data, which included MK-8(H) as the major menaquinone; -diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C, anteiso-C, C and iso-C as major fatty acids, supported the affiliation of strain 2C6-41 to the genus . The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA–DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41 to be differentiated genotypically and phenotypically from recognized species of the genus . The isolate is therefore considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 2C6-41 ( = KCTC 19800 = JCM 17458).

  • Published Online:
Funding
This study was supported by the:
  • GAIA Project, Ministry of Environment, Republic of Korea (Award 173-091-006)
  • MEST (Award 2009-0081153)
© 2013 IUMS
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2013-01-01
2023-09-23
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Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture
Int J Syst Evol Microbiol 63, 152 (2013); https://doi.org/10.1099/ijs.0.038141-0
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