1887

Abstract

A facultative anaerobic, non-motile, non-spore-forming, Gram-positive-staining, coccus-shaped bacterium was isolated from an abscess on the right foot of a chimpanzee (). The colonies were β-haemolytic. Catalase and oxidase activities were negative. The Lancefield group B antigen was expressed. On the basis of morphological and biochemical characteristics, the bacterium was tentatively identified as a streptococcal species. 16S rRNA gene sequence analysis indicated that the bacterium shared 96.7 %, 96.4 %, 96.1 %, 95.8 % and 95.7 % sequence similarities with , , , and respectively. Phylogenetic analyses based on the sequences of the 16S rRNA gene and housekeeping genes encoding -alanine : -alanine ligase (), the β-subunit of RNA polymerase () and manganese-dependent superoxide dismutase ( revealed that the bacterium represented a novel species closely related to, albeit different from, , and the anginosus streptococci. The name sp. nov. is proposed. The type strain is M09-11185 ( = ATCC BAA-2337 = KCTC 33006).

Funding
This study was supported by the:
  • , Mississippi State University
  • , Texas A&M University
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.038133-0
2013-02-01
2020-07-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/449.html?itemId=/content/journal/ijsem/10.1099/ijs.0.038133-0&mimeType=html&fmt=ahah

References

  1. CLSI ( 2008 ). Performance Standards for Antimicrobial Disk Diffusion Susceptibility Tests; Approved Standard Informational Supplement M100-S15. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  2. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral J. P., Raoult D. ( 2000 ). 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. . J Clin Microbiol 38, 36233630.[PubMed]
    [Google Scholar]
  3. Facklam R. ( 2002 ). What happened to the streptococci: overview of taxonomic and nomenclature changes. . Clin Microbiol Rev 15, 613630. [CrossRef] [PubMed]
    [Google Scholar]
  4. Flynn C. E., Ruoff K. L. ( 1995 ). Identification of “Streptococcus milleri” group isolates to the species level with a commercially available rapid test system. . J Clin Microbiol 33, 27042706.[PubMed]
    [Google Scholar]
  5. Gaustad P. ( 1985 ). Genetic transformation in Streptococcus sanguis. Identification, surface spreading and competence of invasive strains of Streptococcus sanguis Lancefield groups H and W and other invasive viridans streptococci. . Acta Pathol Microbiol Immunol Scand [B] 93, 277282.[PubMed]
    [Google Scholar]
  6. Gribaldo S., Philippe H. ( 2002 ). Ancient phylogenetic relationships. . Theor Popul Biol 61, 391408. [CrossRef] [PubMed]
    [Google Scholar]
  7. Hoshino T., Fujiwara T., Kilian M. ( 2005 ). Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients. . J Clin Microbiol 43, 60736085. [CrossRef] [PubMed]
    [Google Scholar]
  8. Huelsenbeck J. P., Ronquist F. ( 2001 ). mrbayes: Bayesian inference of phylogenetic trees. . Bioinformatics 17, 754755. [CrossRef] [PubMed]
    [Google Scholar]
  9. Jacobs J. A., Schot C. S., Schouls L. M. ( 2000 ). The Streptococcus anginosus species comprises five 16S rRNA ribogroups with different phenotypic characteristics and clinical relevance. . Int J Syst Evol Microbiol 50, 10731079. [CrossRef] [PubMed]
    [Google Scholar]
  10. Janda J. M., Abbott S. L. ( 2007 ). 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. . J Clin Microbiol 45, 27612764. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jobb G., von Haeseler A., Strimmer K. ( 2004 ). treefinder: a powerful graphical analysis environment for molecular phylogenetics. . BMC Evol Biol 4, 18. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kawamura Y., Hou X. G., Sultana F., Miura H., Ezaki T. ( 1995 ). Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus . . Int J Syst Bacteriol 45, 406408. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kikuchi K., Enari T., Totsuka K., Shimizu K. ( 1995 ). Comparison of phenotypic characteristics, DNA-DNA hybridization results, and results with a commercial rapid biochemical and enzymatic reaction system for identification of viridans group streptococci. . J Clin Microbiol 33, 12151222.[PubMed]
    [Google Scholar]
  14. Kiratisin P., Li L., Murray P. R., Fischer S. H. ( 2005 ). Use of housekeeping gene sequencing for species identification of viridans streptococci. . Diagn Microbiol Infect Dis 51, 297301. [CrossRef] [PubMed]
    [Google Scholar]
  15. Limia A., Alarcón T., Jiménez M. L., López-Brea M. ( 2000 ). Comparison of three methods for identification of Streptococcus milleri group isolates to species level. . Eur J Clin Microbiol Infect Dis 19, 128131. [CrossRef] [PubMed]
    [Google Scholar]
  16. Nikkari S., Lopez F. A., Lepp P. W., Cieslak P. R., Ladd-Wilson S., Passaro D., Danila R., Relman D. A. ( 2002 ). Broad-range bacterial detection and the analysis of unexplained death and critical illness. . Emerg Infect Dis 8, 188194. [CrossRef] [PubMed]
    [Google Scholar]
  17. Philippe H., Lartillot N., Brinkmann H. ( 2005 ). Multigene analyses of bilaterian animals corroborate the monophyly of Ecdysozoa, Lophotrochozoa, and Protostomia. . Mol Biol Evol 22, 12461253. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [CrossRef] [PubMed]
    [Google Scholar]
  19. Schouls L. M., Schot C. S., Jacobs J. A. ( 2003 ). Horizontal transfer of segments of the 16S rRNA genes between species of the Streptococcus anginosus group. . J Bacteriol 185, 72417246. [CrossRef] [PubMed]
    [Google Scholar]
  20. Summanen P. H., Rowlinson M. C., Wooton J., Finegold S. M. ( 2009 ). Evaluation of genotypic and phenotypic methods for differentiation of the members of the Anginosus group streptococci. . Eur J Clin Microbiol Infect Dis 28, 11231128. [CrossRef] [PubMed]
    [Google Scholar]
  21. Templeton T. J., Enomoto S., Chen W. J., Huang C. G., Lancto C. A., Abrahamsen M. S., Zhu G. ( 2010 ). A genome-sequence survey for Ascogregarina taiwanensis supports evolutionary affiliation but metabolic diversity between a Gregarine and Cryptosporidium . . Mol Biol Evol 27, 235248. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tunkel A. R., Sepkowitz K. A. ( 2002 ). Infections caused by viridans streptococci in patients with neutropenia. . Clin Infect Dis 34, 15241529. [CrossRef] [PubMed]
    [Google Scholar]
  23. Whiley R. A., Beighton D. ( 1991 ). Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus as distinct species. . Int J Syst Bacteriol 41, 15. [CrossRef] [PubMed]
    [Google Scholar]
  24. Whiley R. A., Hardie J. M. ( 2009 ). Genus I. Streptococcus Rosenbach 1884, 22AL . . In Bergey’s Manual of Systematic Bacteriology (The Firmicutes), , 2nd edn., vol. 3, pp. 655711. Edited by deVos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  25. Whiley R. A., Fraser H., Hardie J. M., Beighton D. ( 1990 ). Phenotypic differentiation of Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus strains within the “Streptococcus milleri group”. . J Clin Microbiol 28, 14971501.[PubMed]
    [Google Scholar]
  26. Whiley R. A., Beighton D., Winstanley T. G., Fraser H. Y., Hardie J. M. ( 1992 ). Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus (the Streptococcus milleri group): association with different body sites and clinical infections. . J Clin Microbiol 30, 243244.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.038133-0
Loading
/content/journal/ijsem/10.1099/ijs.0.038133-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error