1887

Abstract

Five yeast strains isolated from plant leaves collected in south-east Tibet formed cream to brownish colonies and produced asymmetrical ballistoconidia and CoQ-9 as the major ubiquinone. Sequence analysis of the 26S rRNA D1/D2 domain and the internal transcribed spacer region indicated that these strains represented two novel species of the genus . The names sp. nov. (type strain XZ 4C5 = CGMCC 2.02635 = CBS 10710) and sp. nov. (type strain XZ 33D1 = CGMCC 2.02670 = CBS 10713) are proposed for the two novel species, which are phylogenetically closely related to , and the type species of the genus, .

Funding
This study was supported by the:
  • , National Natural Science Foundation of China (NSFC) , (Award 31010103902, 30825002 and 30970013)
  • , Knowledge Innovation Program of the Chinese Academy of Sciences , (Award KSCX2-YW-Z-0936)
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2012-08-01
2021-03-08
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References

  1. Bai F. Y., Zhao J. H., Takashima M., Jia J. H., Boekhout T., Nakase T. 2002; Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov.. Int J Syst Evol Microbiol 52:2309–2314 [CrossRef][PubMed]
    [Google Scholar]
  2. Boekhout T., Fonseca A., Sampaio J. P., Bandoni R. J., Fell J. W., Kwon-Chung K. J. 2011; Discussion of teleomorphic and anamorphic basidiomycetous yeasts. In The Yeasts, A Taxonomic Study, 5th edn. pp. 1339–1372 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  3. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Hamamoto M., Nakase T. 2000; Phylogenetic analysis of the ballistoconidium-forming yeast genus Sporobolomyces based on 18S rDNA sequences. Int J Syst Evol Microbiol 50:1373–1380 [CrossRef][PubMed]
    [Google Scholar]
  6. Ingold C. T. 1986; Bensingtonia ciliata gen. et sp. nov., a ballistosporic fungus. Trans Br Mycol Soc 86:325–328 [CrossRef]
    [Google Scholar]
  7. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  8. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, A Taxonomic Study, 5th edn. pp. 87–110 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  9. Makimura K., Murayama S. Y., Yamaguchi H. 1994; Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40:358–364 [CrossRef][PubMed]
    [Google Scholar]
  10. Nakase T., Boekhout T. 1988; Emendation of the genus Bensingtonia Ingold. J Gen Appl Microbiol 34:433–437 [CrossRef]
    [Google Scholar]
  11. Nakase T., Suzuki M. 1986; Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240 [CrossRef]
    [Google Scholar]
  12. Nakase T., Takashima M. 1993; A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Review 3:33–34
    [Google Scholar]
  13. Nakase T., Bai F. Y., Boekhout T. 2011; Bensingtonia Ingold emend. Nakase & Boekhout. In The Yeasts, A Taxonomic Study, 5th edn. pp. 1607–1622 Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  15. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517[PubMed] [CrossRef]
    [Google Scholar]
  16. Takashima M., Suh S. O., Nakase T. 1995; Phylogenetic relationships among species of the genus Bensingtonia and related taxa based on the small subunit ribosomal DNA sequences. J Gen Appl Microbiol 41:131–141 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  18. Wang Q. M., Bai F. Y. 2008; Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage (Tremellales) based on nuclear rRNA and mitochondrial cytochrome b gene sequence analyses: proposal of Derxomyces gen. nov. and Hannaella gen. nov., and description of eight novel Derxomyces species. FEMS Yeast Res 8:799–814 [CrossRef][PubMed]
    [Google Scholar]
  19. Wang Q. M., Bai F. Y., Zhao J. H., Jia J. H. 2003; Bensingtonia changbaiensis sp. nov. and Bensingtonia sorbi sp. nov., novel ballistoconidium-forming yeast species from plant leaves. Int J Syst Evol Microbiol 53:2085–2089 [CrossRef][PubMed]
    [Google Scholar]
  20. Wang Q. M., Jia J. H., Bai F. Y. 2006; Bensingtonia pseudonaganoensis sp. nov., a novel ballistoconidium-forming yeast species isolated from plant leaves. Antonie van Leeuwenhoek 89:261–266 [CrossRef][PubMed]
    [Google Scholar]
  21. Wang Q. M., Bai F. Y., Fungsin B., Boekhout T., Nakase T. 2011; Proposal of Mingxiaea gen. nov. for the anamorphic basidiomycetous yeast species in the Bulleribasidium clade (Tremellales) based on molecular phylogenetic analysis, with six new combinations and four novel species. Int J Syst Evol Microbiol 61:210–219 [CrossRef][PubMed]
    [Google Scholar]
  22. Yamada Y., Kondo K. 1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast like genera Sporobolomyces and Rhodosporidium . J Gen Appl Microbiol 19:59–77 [CrossRef]
    [Google Scholar]
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