1887

Abstract

Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT-17 and RHLT-1) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with KSL-2. Strain RHLT-17 grew at 0–35 °C and strain RHLT-1 grew at 0–25 °C. The major cellular fatty acids of strain RHLT-17 were Cω8 (32.69 %) and iso-C (21.74 %). The major cellular fatty acids of strain RHLT-1 were Cω9 (28.72 %), summed feature 3 (17.14 %; comprising Cω7 and/or Cω6), iso-C (14.35 %), C (9.96 %) and iso-C (8.34 %). Both strains contained -2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, sp. nov. (type strain RHLT-17  = CGMCC 1.11147  = NBRC 108562) and sp. nov. (type strain RHLT-1  = CGMCC 1.11156  = NBRC 108563), are proposed.

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2013-01-01
2019-10-21
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