1887

Abstract

Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT-17 and RHLT-1) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with KSL-2. Strain RHLT-17 grew at 0–35 °C and strain RHLT-1 grew at 0–25 °C. The major cellular fatty acids of strain RHLT-17 were Cω8 (32.69 %) and iso-C (21.74 %). The major cellular fatty acids of strain RHLT-1 were Cω9 (28.72 %), summed feature 3 (17.14 %; comprising Cω7 and/or Cω6), iso-C (14.35 %), C (9.96 %) and iso-C (8.34 %). Both strains contained -2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, sp. nov. (type strain RHLT-17  = CGMCC 1.11147  = NBRC 108562) and sp. nov. (type strain RHLT-1  = CGMCC 1.11156  = NBRC 108563), are proposed.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31070001)
  • , Knowledge Innovation Project of the Chinese Academy of Sciences , (Award KSCXZ-YW-Z-0937)
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2013-01-01
2020-08-11
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References

  1. Cho C. H., Lee J.-S., An D.-S., Whon T. W., Kim S.-G. ( 2010 ). Nocardioides panacisoli sp. nov., isolated from the soil of a ginseng field. . Int J Syst Evol Microbiol 60, 387392. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. Dastager S. G., Lee J.-C., Ju Y.-J., Park D.-J., Kim C.-J. ( 2009 ). Nocardioides sediminis sp. nov., isolated from a sediment sample. . Int J Syst Evol Microbiol 59, 280284. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Euzéby J. P. ( 1997 ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590592. http://www.bacterio.cict.fr [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strain. . Int J Syst Bacteriol 24, 5463. [CrossRef]
    [Google Scholar]
  9. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319322. [CrossRef]
    [Google Scholar]
  10. Kim H. M., Choi D. H., Hwang C. Y., Cho B. C. ( 2008 ). Nocardioides salarius sp. nov., isolated from seawater enriched with zooplankton. . Int J Syst Evol Microbiol 58, 20562064. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim K. H., Roh S. W., Chang H. W., Nam Y.-D., Yoon J.-H., Jeon C. O., Oh H.-M., Bae J. W. ( 2009 ). Nocardioides basaltis sp. nov., isolated from black beach sand. . Int J Syst Evol Microbiol 59, 4247. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299306. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lee D. W., Hyun C.-G., Lee S. D. ( 2007 ). Nocardioides marinisabuli sp. nov., a novel actinobacterium isolated from beach sand. . Int J Syst Evol Microbiol 57, 29602963. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lee S.-H., Liu Q.-M., Lee S.-T., Kim S.-C., Im W.-T. ( 2012 ). Nocardioides ginsengagri sp. nov., isolated from the soil of a ginseng field. . Int J Syst Evol Microbiol 62, 591595. [CrossRef] [PubMed]
    [Google Scholar]
  15. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [CrossRef] [PubMed]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [CrossRef]
    [Google Scholar]
  17. Prauser H. ( 1976 ). Nocardioides, a new genus of the order Actinomycetales . . Int J Syst Bacteriol 26, 5865. [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  20. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tóth E. M., Kéki Z., Makk J., Homonnay Z. G., Márialigeti K., Schumann P. ( 2011 ). Nocardioides hungaricus sp. nov., isolated from a drinking water supply system. . Int J Syst Evol Microbiol 61, 549553. [CrossRef] [PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  24. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176178. [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Rhee S.-K., Lee J.-S., Park Y.-H., Lee S. T. ( 1997 ). Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. . Int J Syst Bacteriol 47, 933938. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. ( 2004 ). Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. . Int J Syst Evol Microbiol 54, 7175. [CrossRef] [PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kim I.-G., Lee M.-H., Oh T.-K. ( 2005 ). Nocardioides kribbensis sp. nov., isolated from an alkaline soil. . Int J Syst Evol Microbiol 55, 16111614. [CrossRef] [PubMed]
    [Google Scholar]
  28. Yoon J.-H., Park S., Kang S.-J., Lee J.-S., Lee K. C., Oh T.-K. ( 2010 ). Nocardioides daedukensis sp. nov., a halotolerant bacterium isolated from soil. . Int J Syst Evol Microbiol 60, 13341338. [CrossRef] [PubMed]
    [Google Scholar]
  29. Zhang D.-C., Schumann P., Redzic M., Zhou Y.-G., Liu H.-C., Schinner F., Margesin R. ( 2012 ). Nocardioides alpinus sp. nov., a psychrophilic actinomycete isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol 62, 445450. [CrossRef] [PubMed]
    [Google Scholar]
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